STUDY OF FRACTIONATION CAPACITY ADDITION ON TOXOID TETANUS PURIFICATION USING FRACTIONAL FACTORIAL DESIGN
BFM is a pharmaceutical company that that produces purified tetanus toxoid. The demand for BFM’s purified tetanus toxoid will continue to increase until 2022, while the occupied production capacity in 2018 has reached 82%. One effort to increase the production capacity of purified tetanus toxoi...
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| 主要作者: | |
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| 格式: | Theses |
| 語言: | Indonesia |
| 在線閱讀: | https://digilib.itb.ac.id/gdl/view/40469 |
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| 機構: | Institut Teknologi Bandung |
| 語言: | Indonesia |
| 總結: | BFM is a pharmaceutical company that that produces purified tetanus toxoid. The
demand for BFM’s purified tetanus toxoid will continue to increase until 2022,
while the occupied production capacity in 2018 has reached 82%. One effort to
increase the production capacity of purified tetanus toxoid is fractionation capacity
addition on purification process. This study aims to determine and analyze the
influential factors and interactions that affect the yield and purity as a quantitative
response to the fractionation process. The results of the analysis were used as a
evaluating basis for plans of fractionation capacity addition in the process of
tetanus toxoid purification. Influential factors are determined by the fishbone and
failure mode and effect analysis (FMEA) methods. The fractional factorial of design
of experiments was carried out on four influential factors, namely: titers,
sedimentation time, centrifugation time, and centrifugation speed. The response of
yield and purity in the design of experiments was analyzed using analysis of
variance (ANOVA), pareto graph, factorial plots, and response optimizer. The
results of the analysis show the four factors and their interactions have a significant
effect (p-value <0.05), have a positive effect, and the curvature relationship is
significant towards the yield. Titers, centrifugation times and their interactions, and
interactions between titers with centrifugation speed have a significant effect (pvalue <0.05), have a positive effect, and curvature relationships are not significant
for purity. All experimental treatments produce products according to the
specifications of purified tetanus toxoid. Based on fractional factorial analysis, the
the capacity addition of the tetanus toxoid purification process can be done by
fractionation of concentration titer at a 3000 Lf/ml. With the increase in the
capacity of the fractionation process, actual production capacity will increase by
around 1.5 times the dose and the occupied production capacity decreases to 55%.
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