FORMULATION OF NANOSTRUCTURED LIPID CARRIERS LOADED WITH PACAR AIR HERB ETHANOL EXTRACT (IMPATIENS BALSAMINA L.) TO ENHANCE ANTIINFLAMATORY ACTIVITY IN DERMATITIS
The pacar air plant (Impatiens balsamina L.) is widely cultivated in Bali dan possesses antipruritic, anti-irritant, dan anti-inflammatory activities. However, the direct use of balsam plant extract to reduce inflammation symptoms has shown less favorable results. This study aims to develop a Nanost...
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| 格式: | Theses |
| 語言: | Indonesia |
| 在線閱讀: | https://digilib.itb.ac.id/gdl/view/82644 |
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| 總結: | The pacar air plant (Impatiens balsamina L.) is widely cultivated in Bali dan possesses antipruritic, anti-irritant, dan anti-inflammatory activities. However, the direct use of balsam plant extract to reduce inflammation symptoms has shown less favorable results. This study aims to develop a Nanostructured Lipid Carrier (NLC) containing ethanol extract of balsam herb (NLC-IBE) dan to determine its anti-inflammatory activity both in vitro dan in vivo. NLC-IBE was prepared using an optimized high shear homogenization and ultrasonication method based on Box-Behnken Design variables: total lipid concentration, probe ultrasonication time, dan ultra turrax homogenization speed. The in vitro anti-inflammatory activity study was conducted by testing the inhibition of Bovine Serum Albumin (BSA) denaturation. Additionally, the in vivo anti-inflammatory activity was tested on an allergic contact dermatitis (ACD) animal model induced by 2,4-dinitrofluorobenzene (DNFB). The optimal NLC-IBE formulation consisted of 0.5% (w/v) IBE, a combination of black cumin oil dan stearyl alcohol (4:6) at 3.75% (w/v), Span®20 : Plantacare® 1200 surfactants (4:1) at 5% (w/v), homogenized at 6600 rpm for 8 minutes, dan probe ultrasonicated at 70% for 5 minutes. The optimal NLC-IBE formulation resulted in particle size, polydispersity index, entrapment efficiency, dan zeta potential of 194.47 ± 7.96 nm, 0.24 ± 0.01, 94.92 ± 0.99%, dan -40.78 ± 4.73 mV respectively, with spherical or oval morphology dan stability over 28 days at 4°C. In vitro drug release tests showed that lawson release from the optimal NLC-IBE formulation followed a biphasic pattern with a cumulative release of 71.043 ± 1.46% over 24 hours. The IC50 value of the optimal NLC-IBE in the BSA denaturation inhibition test was 109.56 ± 3.95 µg/mL. In vivo, NLC-IBE showed erythema scores (0.57 ± 1.13 mm), ear thickness (0.25 ± 0.01 mm), and skin moisture percentage (37.24 ± 2.90%) in the ACD animal model significantly (p value < 0.05) compared to ethanol extract of henna herb on the 4th day of observation after drug administration. Overall, the optimized NLC-IBE produced can enhance the anti-inflammatory activity of the ethanol extract of henna herb (IBE).
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