ANTIGENITAS PROTEIN Toxocara vitulorum DENGAN SERUM ANTI-Mecistocirrus digitatus MENGGUNAKAN TEKNIK WESTERN BLOT
The purpose of this research to get the specific protein from adult stage of Toxocara vitulorum used Whole Worm Extract (WWE) as antigen which can be used for toxocariasis diagnostic serologically. Toxocara vitulorum was collected from gut cattle. To homogenize WWE, mortar and stamfer are used, t...
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Main Author: | |
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Format: | Theses and Dissertations NonPeerReviewed |
Language: | English Indonesian |
Published: |
2017
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Subjects: | |
Online Access: | http://repository.unair.ac.id/60934/1/ABSTRACT%20KKC%20KK%20KH.%20172.17%20Ang%20a.pdf http://repository.unair.ac.id/60934/2/FULLTEXT%20KKC%20KK%20KH.%20172.17%20Ang%20a.pdf http://repository.unair.ac.id/60934/ http://lib.unair.ac.id |
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Institution: | Universitas Airlangga |
Language: | English Indonesian |
Summary: | The purpose of this research to get the specific protein from adult stage of
Toxocara vitulorum used Whole Worm Extract (WWE) as antigen which can be
used for toxocariasis diagnostic serologically. Toxocara vitulorum was collected
from gut cattle. To homogenize WWE, mortar and stamfer are used, then added
by 2 ml of PBS, next WWE homogenized by centrifuge 4.000 rpm while 15
minutes. Initiation and booster were done to get policlonal antibody from mice.
Indirect ELISA used to measure sera antibody levels. Separation of protein by
discontinuous system of SDS-PAGE technique (12% separating gel and 5%
stacking gel). Characterization of specific protein by semi dry of Western blot
technique with western blue staining used sera anti-Mecistocirrus digitatus and
IgG anti-mouse. The result of this research are the protein’s bands which each of
them have molecular weight. The result showed the existence of cross-reaction
between WWE T. vitulorum antigen with anti-M. digitatus serum in a protein
with a molecular weight of 176, 124, 92, 68, 59, 47, 31, 29, 26, 16, 12 and 10
kDa. |
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