Toll-Like Receptor (TLRs) Play Role in Adaptive Immunity in Rabbits Immunized by Sarcoptes scabiei Protein
Sarcoptic mange is one of the most economically important diseases in goats in Indonesia, and increasing number of cases of treatment failure is being reported because of drug resistance. Nowadays, it is considered as an emerging/re-emerging parasitic disease that threatens human and animal health g...
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Faculty of Veterinary Medicine University Airlangga
2017
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id-langga.988292020-09-16T02:37:05Z http://repository.unair.ac.id/98829/ Toll-Like Receptor (TLRs) Play Role in Adaptive Immunity in Rabbits Immunized by Sarcoptes scabiei Protein Nunuk Dyah Retno Lastuti Fedik Abdul Rantam Poedji Hastutiek Dony Chrismanto SF451-455 Rabbits and hares SF811-909 Veterinary medicine of special organs, regions, and systems Sarcoptic mange is one of the most economically important diseases in goats in Indonesia, and increasing number of cases of treatment failure is being reported because of drug resistance. Nowadays, it is considered as an emerging/re-emerging parasitic disease that threatens human and animal health globally. Toll-Like Receptors (TLR) is a receptor which plays role in innate immunity due to microbial infection. TLR-5 is a receptor that can recognize ligand produced by bacterial component, and TLR-7 is involved in the recognition of ligand that similar to ssRNA virus. This research aims are to detect Sarcoptes scabiei antigenic protein which can induce cellular immune response in rabbit as adaptive immunity with TLR-5 and TLR-7 as marker.This research was performed in several stages: isolation and identification S. scabiei from scabies infected goats; extraction of soluble protein S.scabiei mite, the rabbit immunization by inoculating protein antigen S. scabiei with dosage of 500μg, repeated five times as booster in two weeks, the examination of TLR-5 and TLR-7 expression using direct immunofluorescence technique. The result of cellular immune response is shown by TLR-5 and TLR-7 expression in rabbit T limphocytes which appear yellow to green fluorescence color using fluorescence microscope. The amount of fluoresnce T lymphocytes showed a significant difference (p<0.05) between control and various boosters, and significantly increased in 3rd booster or 42 days post immunization. The conclusion showed protein from S. scabiei mites taken from goat contains ligands, acting as receptors which involve in pathogen associated molecular pattern (PAMP) that can induce adaptive immunity and recognized by TLR-5 and TLR-7. It shows that TLR is not only involved in innate immunity but also in adaptive immunity, and can be used as alternative adjuvant development. Keywords: Sarcoptes scabiei, TLR- 5, TLR-7, goats, rabbits Faculty of Veterinary Medicine University Airlangga 2017-07 Article PeerReviewed text en http://repository.unair.ac.id/98829/1/Bukti%20C%2025%20Toll-Like%20Receptor%20%28TLRs%29%20Play%20Role%20..._compressed.pdf text en http://repository.unair.ac.id/98829/2/Peer%20review%20C%2025%20Toll-Like%20Receptor%20%28TLRs%29%20Play%20Role%20in%20....pdf text en http://repository.unair.ac.id/98829/3/25.%20Toll-Like%20Receptors%20%28TLRs%29%20Play%20Role%20in%20Adaptive%20....pdf Nunuk Dyah Retno Lastuti and Fedik Abdul Rantam and Poedji Hastutiek and Dony Chrismanto (2017) Toll-Like Receptor (TLRs) Play Role in Adaptive Immunity in Rabbits Immunized by Sarcoptes scabiei Protein. Proceeding The Veterinary Medicine International Conference (VMIC 2017). pp. 1-9. ISSN 2413-0877 https://knepublishing.com/index.php/Kne-Life/article/view/1100 |
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SF451-455 Rabbits and hares SF811-909 Veterinary medicine of special organs, regions, and systems Nunuk Dyah Retno Lastuti Fedik Abdul Rantam Poedji Hastutiek Dony Chrismanto Toll-Like Receptor (TLRs) Play Role in Adaptive Immunity in Rabbits Immunized by Sarcoptes scabiei Protein |
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Sarcoptic mange is one of the most economically important diseases in goats in Indonesia, and increasing number of cases of treatment failure is being reported because of drug resistance. Nowadays, it is considered as an emerging/re-emerging parasitic disease that threatens human and animal health globally. Toll-Like Receptors (TLR) is a receptor which plays role in innate immunity due to microbial infection. TLR-5 is a receptor that can recognize ligand produced by bacterial component, and TLR-7 is involved in the recognition of ligand that similar to ssRNA virus. This research aims are to detect Sarcoptes scabiei antigenic protein which can induce cellular immune response in rabbit as adaptive immunity with TLR-5 and TLR-7 as marker.This research was performed in several stages: isolation and identification S. scabiei from scabies infected goats; extraction of soluble protein S.scabiei mite, the rabbit immunization by inoculating protein antigen S. scabiei with dosage of 500μg, repeated five times as booster in two weeks, the examination of TLR-5 and TLR-7 expression
using direct immunofluorescence technique. The result of cellular immune response is shown by TLR-5 and TLR-7 expression in rabbit T limphocytes which appear yellow to green fluorescence color using fluorescence microscope. The amount of fluoresnce
T lymphocytes showed a significant difference (p<0.05) between control and various boosters, and significantly increased in 3rd booster or 42 days post immunization. The conclusion showed protein from S. scabiei mites taken from goat contains ligands, acting as receptors which involve in pathogen associated molecular pattern (PAMP) that can induce adaptive immunity and recognized by TLR-5 and TLR-7. It shows that TLR is not only involved in innate immunity but also in adaptive immunity, and can be used as alternative adjuvant development.
Keywords: Sarcoptes scabiei, TLR- 5, TLR-7, goats, rabbits |
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Article PeerReviewed |
author |
Nunuk Dyah Retno Lastuti Fedik Abdul Rantam Poedji Hastutiek Dony Chrismanto |
author_facet |
Nunuk Dyah Retno Lastuti Fedik Abdul Rantam Poedji Hastutiek Dony Chrismanto |
author_sort |
Nunuk Dyah Retno Lastuti |
title |
Toll-Like Receptor (TLRs) Play Role in Adaptive Immunity in Rabbits Immunized by Sarcoptes scabiei Protein |
title_short |
Toll-Like Receptor (TLRs) Play Role in Adaptive Immunity in Rabbits Immunized by Sarcoptes scabiei Protein |
title_full |
Toll-Like Receptor (TLRs) Play Role in Adaptive Immunity in Rabbits Immunized by Sarcoptes scabiei Protein |
title_fullStr |
Toll-Like Receptor (TLRs) Play Role in Adaptive Immunity in Rabbits Immunized by Sarcoptes scabiei Protein |
title_full_unstemmed |
Toll-Like Receptor (TLRs) Play Role in Adaptive Immunity in Rabbits Immunized by Sarcoptes scabiei Protein |
title_sort |
toll-like receptor (tlrs) play role in adaptive immunity in rabbits immunized by sarcoptes scabiei protein |
publisher |
Faculty of Veterinary Medicine University Airlangga |
publishDate |
2017 |
url |
http://repository.unair.ac.id/98829/1/Bukti%20C%2025%20Toll-Like%20Receptor%20%28TLRs%29%20Play%20Role%20..._compressed.pdf http://repository.unair.ac.id/98829/2/Peer%20review%20C%2025%20Toll-Like%20Receptor%20%28TLRs%29%20Play%20Role%20in%20....pdf http://repository.unair.ac.id/98829/3/25.%20Toll-Like%20Receptors%20%28TLRs%29%20Play%20Role%20in%20Adaptive%20....pdf http://repository.unair.ac.id/98829/ https://knepublishing.com/index.php/Kne-Life/article/view/1100 |
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