The Potential of Antigenic of Sarcoptes scabiei as a Serological Diagnostic Candidate for Scabies in Goat
In Indonesia, the prevalence of scabies on goat cattle is still high, which shows that scabies is still not handled properly. It is now considered as an emerging/re-emerging parasitic disease that threatens human and animal health globally. The scabies disease has been known for a thousand years, an...
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UNAIR-Universitas Lambung Mangkurat-Universitas Mataram
2018
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id-langga.989012020-09-16T02:39:49Z http://repository.unair.ac.id/98901/ The Potential of Antigenic of Sarcoptes scabiei as a Serological Diagnostic Candidate for Scabies in Goat Nunuk Dyah Retno Lastuti Dony Chrismanto Poedji Hastutiek Agus Sunarso SF380-388 Goats SF811-909 Veterinary medicine of special organs, regions, and systems In Indonesia, the prevalence of scabies on goat cattle is still high, which shows that scabies is still not handled properly. It is now considered as an emerging/re-emerging parasitic disease that threatens human and animal health globally. The scabies disease has been known for a thousand years, and it is a persistent problem on public health and also livestock. Yet, until today a serological diagnostic tool is sensitive and specific is not available, especially for goat cattle in Indonesia. In recent years, an indirect antibody enzymelinked immunosorbent assay (ELISA) has been available, which has higher sensitivity and specificity than traditional diagnostic methods. In order to overcome those problems preventive actions need to be undertaken through research for serological diagnostic development as an alternative for scabies prevention in goats in Indonesia. The purpose of this research is doing sensitivity and specificity tests towards the antigenic protein of Sarcoptes scabiei isolated from goat as diagnostic material for scabies in goats. The method was comprised of deciding the gold standard for positive and negative scabies, checkerboard test toward antigenic protein, and ELISA test for measuring sensitivity and specificity. The research results showed that S. scabiei antigenic protein with molecular weight of 57.3 kDa can be recognized by serum antibody of goat that infested with scabies with sensitivity level of 96% and specificity of 86,6%. From that result, it can be recommended that S.scabiei with a molecular weight of 57.3 kDa is the specific antigenic protein that can be used as a candidate of serological diagnostic material for scabies isolated from Indonesian local goat. Keywords: Antigenic Protein, Goat, Sarcoptes scabiei, Serological diagnostic UNAIR-Universitas Lambung Mangkurat-Universitas Mataram 2018 Article PeerReviewed text en http://repository.unair.ac.id/98901/2/Bukti%20C%2027%20The%20%20Potential%20of%20Antigenic%20of%20Sarcoptes%20....pdf text en http://repository.unair.ac.id/98901/1/Peer%20review%20C%2027%20The%20%20Potential%20of%20Antigenic%20of%20Sarcoptes%20....pdf text en http://repository.unair.ac.id/98901/3/27.%20The%20Potential%20of%20Antigenic%20Protein%20of%20Sarcoptes%20scabiei%20....pdf Nunuk Dyah Retno Lastuti and Dony Chrismanto and Poedji Hastutiek and Agus Sunarso (2018) The Potential of Antigenic of Sarcoptes scabiei as a Serological Diagnostic Candidate for Scabies in Goat. Proceeding 2nd International Conference Postgradute School (ICPS 2018). pp. 537-540. ISSN 978-989-758-348-3 http://www.scitepress.org/DigitalLibrary/Link.aspx?doi=10.5220/0007546505370540 |
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SF380-388 Goats SF811-909 Veterinary medicine of special organs, regions, and systems Nunuk Dyah Retno Lastuti Dony Chrismanto Poedji Hastutiek Agus Sunarso The Potential of Antigenic of Sarcoptes scabiei as a Serological Diagnostic Candidate for Scabies in Goat |
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In Indonesia, the prevalence of scabies on goat cattle is still high, which shows that scabies is still not handled properly. It is now considered as an emerging/re-emerging parasitic disease that threatens human and animal health globally. The scabies disease has been known for a thousand years, and it is a persistent problem on public health and also livestock. Yet, until today a serological diagnostic tool is sensitive and specific is not available, especially for goat cattle in Indonesia. In recent years, an indirect antibody enzymelinked immunosorbent assay (ELISA) has been available, which has higher sensitivity and specificity than traditional diagnostic methods. In order to overcome those problems preventive actions need to be undertaken through research for serological diagnostic development as an alternative for scabies prevention in goats in Indonesia. The purpose of this research is doing sensitivity and specificity tests towards the antigenic protein of Sarcoptes scabiei isolated from goat as diagnostic material for scabies in goats. The method was comprised of deciding the gold standard for positive and negative scabies, checkerboard test toward antigenic protein, and ELISA test for measuring sensitivity and specificity. The research results showed that S. scabiei antigenic protein with molecular weight of 57.3 kDa can be recognized by serum antibody of goat that infested with scabies with sensitivity level of 96% and specificity of 86,6%. From that result, it can be recommended that S.scabiei with a molecular weight of 57.3 kDa is the specific antigenic protein that can be used as a candidate of serological diagnostic material for scabies isolated from Indonesian local goat.
Keywords: Antigenic Protein, Goat, Sarcoptes scabiei, Serological diagnostic |
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Article PeerReviewed |
author |
Nunuk Dyah Retno Lastuti Dony Chrismanto Poedji Hastutiek Agus Sunarso |
author_facet |
Nunuk Dyah Retno Lastuti Dony Chrismanto Poedji Hastutiek Agus Sunarso |
author_sort |
Nunuk Dyah Retno Lastuti |
title |
The Potential of Antigenic of Sarcoptes scabiei as a Serological Diagnostic Candidate for Scabies in Goat |
title_short |
The Potential of Antigenic of Sarcoptes scabiei as a Serological Diagnostic Candidate for Scabies in Goat |
title_full |
The Potential of Antigenic of Sarcoptes scabiei as a Serological Diagnostic Candidate for Scabies in Goat |
title_fullStr |
The Potential of Antigenic of Sarcoptes scabiei as a Serological Diagnostic Candidate for Scabies in Goat |
title_full_unstemmed |
The Potential of Antigenic of Sarcoptes scabiei as a Serological Diagnostic Candidate for Scabies in Goat |
title_sort |
potential of antigenic of sarcoptes scabiei as a serological diagnostic candidate for scabies in goat |
publisher |
UNAIR-Universitas Lambung Mangkurat-Universitas Mataram |
publishDate |
2018 |
url |
http://repository.unair.ac.id/98901/2/Bukti%20C%2027%20The%20%20Potential%20of%20Antigenic%20of%20Sarcoptes%20....pdf http://repository.unair.ac.id/98901/1/Peer%20review%20C%2027%20The%20%20Potential%20of%20Antigenic%20of%20Sarcoptes%20....pdf http://repository.unair.ac.id/98901/3/27.%20The%20Potential%20of%20Antigenic%20Protein%20of%20Sarcoptes%20scabiei%20....pdf http://repository.unair.ac.id/98901/ http://www.scitepress.org/DigitalLibrary/Link.aspx?doi=10.5220/0007546505370540 |
_version_ |
1681154022996705280 |