STUDY OF AMINOGLYCOSIDES CLASS ANTIBIOTICS SUSCEPTIBILITY TESTING ON METALLO β-LACTAMASE (MBL) PRODUCING Pseudomonas aeruginosa IN CLINICAL ISOLATES FROM DEPARTMENTOFMICROBIOLOGY,FACULTY OF MEDICINEUNIVERSITAS GADJAH MADA

• Main Authors: , RANJIDAM RAMASAMY, , Dr.Nurrokhman., M.S.i.
• Format: Theses and Dissertations NonPeerReviewed
• Published: [Yogyakarta] : Universitas Gadjah Mada 2013
• Subjects: ETD
• Online Access: https://repository.ugm.ac.id/124057/; http://etd.ugm.ac.id/index.php?mod=penelitian_detail&sub=PenelitianDetail&act=view&typ=html&buku_id=64176
• Institution: Universitas Gadjah Mada

Background: Pseudomonas aeruginosais a popular nosocomial pathogen, which is also causes oppotunistic infection. Many P aeruginosaare intrinsically resistant to many antimicrobial therapies making it difficult to treat certain diseases. Mostly they are found to have metallo-β-lactamase(MBL), as one the mechanism for resistance.MBL mediated resistance in P aeruginosahave been increasing since year 2000, making treatment choices very limited in Asia, Europe and Latin America to non-β-Lactams antibiotics. Objectives: The objective of this study is to find prevalence MBL in Pseudomonas aeruginosa and test their sensitivity towards Aminoglycoside class antibiotics and also determine MDR among the isolates. Method: This study is an experimental based descriptive study. 25 isolates of Pseudomonas aeruginosawere obtained from Microbiology Department, UGM, were tested for MBL, using Kirby Baeur disk diffusion test (Imipenem and meropenem antibiotic susceptibility testing) and Combined Disk Imipenem-EDTA method as a confirmatory test. Later Aminoglycoside class antibiotic susceptibility was carried out. Results: Among 25 isolates of Pseudomonas aeruginosa, 4 isolates were screened positive for MBL, later was confirmatory test proved only 3 isolates were MBL postive, 12%.Susceptibility testing showed, 23 isolates were resistant to kananmycin. Three isolates were also found to be multidrug resiatnt, one MBL producing ioslate is MDR. Conclusion:Only 12% MBL found in 25 isolates, all 3 MBL producing isolate were resistant to kananmycin and 3 isolates were found to be MDR.Gentamicin, Amikacin and Tobramycin still has high susceptibility to the isolates tested.