PERBEDAAN PROPORSI POLIMORFISME I/D GENA VEGF (-2549) PADA PENDERITA SIROSIS HATI BERDASARKAN SKOR CHILD PUGH TURCOTTE
Background: Angiogenesis has a very important role on the development of liver cirrhosis. One of the specific stimulator of endothelial target cell in angiogenesis is VEGF (Vascular Endothelial Growth Factor). The common forms of VEGF gene polymorphisms are Single Nucleated Polymorphisms (SNP) or In...
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| 格式: | Theses and Dissertations NonPeerReviewed |
| 出版: |
[Yogyakarta] : Universitas Gadjah Mada
2014
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| 在線閱讀: | https://repository.ugm.ac.id/128061/ http://etd.ugm.ac.id/index.php?mod=penelitian_detail&sub=PenelitianDetail&act=view&typ=html&buku_id=68389 |
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| 總結: | Background: Angiogenesis has a very important role on
the development of liver cirrhosis. One of the specific
stimulator of endothelial target cell in angiogenesis
is VEGF (Vascular Endothelial Growth Factor). The
common forms of VEGF gene polymorphisms are Single
Nucleated Polymorphisms (SNP) or Insertion/Deletion
(I/D). The research VEGF gene polymorphisms in patient
with liver cirrhosis has not been established.
Objective: to discover the difference in the proportion
of I/D VEGF gene -2549 polymorphisms in patient with
liver cirrhosis based on Child-Turcotte Pugh score.
Method: This study uses cross sectional method with
consecutive sampling. The total subject of the research
is 38 patient with liver cirrhosis. 3cc of EDTA blood
is collected as a sample from each patient. The sample
would be examined in biochemistry laboratorium to know
the proportion of I/D gene VEGF polymorphisms. The
methods are DNA isolation, DNA amplification with
Polymerase Chain Reaction (PCR), and electrophoresis.
Kolmogorov-Smirnov test is conducted to investigate the
significancy of difference in the proportion of I/D
VEGF gene -2549 polymorphisms in patient with liver
cirrhosis based on Child- Pugh Turcotte score.
Result: The proportion of CPT A with DD genotype is 4
patients (10,5%), ID 5 patient (13%), and II 6 patients
(15,9%). CPT B with genotype DD is 12 patients (31,6%),
ID 6 patient (15,9%), and 3 patients (7,9%). CPT C with
genotype DD is 6 patient (15,9%), II 1 patient
(2,7%)and none CPT C patient with genotype ID.
Significancy for CPT A with CPT B is 0,989, CPT A with
CPT C is 0,356 and CPT B with CPT C is 0,785.
Conclusion: There is no difference in the proportion
of I/D VEGF gene -2549 polymorphisms in patient with
liver cirrhosis based on Child-Turcotte Pugh score. |
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