Apoptosis induction, cell cycle arrest and in vitro anticancer activity of gonothalamin in a cancer cell lines
Cancer is one of the major health problems worldwide and its current treatments have a number of undesired adverse side effects. Natural compounds may reduce these. Currently, a few plant products are being used to treat cancer. In this study, goniothalamin, a natural occurring styryl-lactone extr...
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| Format: | Article |
| Language: | English English |
| Published: |
2012
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| Subjects: | |
| Online Access: | http://eprints.unisza.edu.my/2448/1/FH02-FPBSM-14-00629.pdf http://eprints.unisza.edu.my/2448/2/FH02-FPBSM-14-01142.jpg http://eprints.unisza.edu.my/2448/ |
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| Institution: | Universiti Sultan Zainal Abidin |
| Language: | English English |
| Summary: | Cancer is one of the major health problems worldwide and its current treatments have a number of undesired
adverse side effects. Natural compounds may reduce these. Currently, a few plant products are being used to
treat cancer. In this study, goniothalamin, a natural occurring styryl-lactone extracted from Goniothalamus
macrophyllus, was investigated for cytotoxic properties against cervical cancer (HeLa), breast carcinoma
(MCF-7) and colon cancer (HT29) cells as well as normal mouse fibroblast (3T3) using MTT assay. Fluorescence
microscopy showed that GTN is able to induce apoptosis in HeLa cells in a time dependent manner. Flow cytometry
further revealed HeLa cells treated with GTN to be arrested in the S phase. Phosphatidyl serine properties
present during apoptosis enable early detection of the apoptosis in the cells. Using annexin V/PI double staining
it could be shown that GTN induces early apoptosis on HeLa cells after 24, 48 and 72 h. It could be concluded
that goniothalamin showing a promising cytotoxicity effect against several cancer cell lines including cervical
cancer cells (HeLa) with apoptosis as the mode of cell death induced on HeLa cells by Goniothalamin was |
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