Apoptosis induction, cell cycle arrest and in vitro anticancer activity of gonothalamin in a cancer cell lines

Cancer is one of the major health problems worldwide and its current treatments have a number of undesired adverse side effects. Natural compounds may reduce these. Currently, a few plant products are being used to treat cancer. In this study, goniothalamin, a natural occurring styryl-lactone extr...

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Main Author:	Manaf, Ali
Format:	Article
Language:	English English
Published:	2012
Subjects:	Q Science (General)
Online Access:	http://eprints.unisza.edu.my/2448/1/FH02-FPBSM-14-00629.pdf http://eprints.unisza.edu.my/2448/2/FH02-FPBSM-14-01142.jpg http://eprints.unisza.edu.my/2448/
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Institution:	Universiti Sultan Zainal Abidin
Language:	English English

id	my-unisza-ir.2448
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spelling	my-unisza-ir.24482021-08-21T08:25:55Z http://eprints.unisza.edu.my/2448/ Apoptosis induction, cell cycle arrest and in vitro anticancer activity of gonothalamin in a cancer cell lines Manaf, Ali Q Science (General) Cancer is one of the major health problems worldwide and its current treatments have a number of undesired adverse side effects. Natural compounds may reduce these. Currently, a few plant products are being used to treat cancer. In this study, goniothalamin, a natural occurring styryl-lactone extracted from Goniothalamus macrophyllus, was investigated for cytotoxic properties against cervical cancer (HeLa), breast carcinoma (MCF-7) and colon cancer (HT29) cells as well as normal mouse fibroblast (3T3) using MTT assay. Fluorescence microscopy showed that GTN is able to induce apoptosis in HeLa cells in a time dependent manner. Flow cytometry further revealed HeLa cells treated with GTN to be arrested in the S phase. Phosphatidyl serine properties present during apoptosis enable early detection of the apoptosis in the cells. Using annexin V/PI double staining it could be shown that GTN induces early apoptosis on HeLa cells after 24, 48 and 72 h. It could be concluded that goniothalamin showing a promising cytotoxicity effect against several cancer cell lines including cervical cancer cells (HeLa) with apoptosis as the mode of cell death induced on HeLa cells by Goniothalamin was 2012 Article NonPeerReviewed text en http://eprints.unisza.edu.my/2448/1/FH02-FPBSM-14-00629.pdf image en http://eprints.unisza.edu.my/2448/2/FH02-FPBSM-14-01142.jpg Manaf, Ali (2012) Apoptosis induction, cell cycle arrest and in vitro anticancer activity of gonothalamin in a cancer cell lines. Asian Pacific journal of cancer prevention. pp. 5131-5136. ISSN 15137368
institution	Universiti Sultan Zainal Abidin
building	UNISZA Library
collection	Institutional Repository
continent	Asia
country	Malaysia
content_provider	Universiti Sultan Zainal Abidin
content_source	UNISZA Institutional Repository
url_provider	https://eprints.unisza.edu.my/
language	English English
topic	Q Science (General)
spellingShingle	Q Science (General) Manaf, Ali Apoptosis induction, cell cycle arrest and in vitro anticancer activity of gonothalamin in a cancer cell lines
description	Cancer is one of the major health problems worldwide and its current treatments have a number of undesired adverse side effects. Natural compounds may reduce these. Currently, a few plant products are being used to treat cancer. In this study, goniothalamin, a natural occurring styryl-lactone extracted from Goniothalamus macrophyllus, was investigated for cytotoxic properties against cervical cancer (HeLa), breast carcinoma (MCF-7) and colon cancer (HT29) cells as well as normal mouse fibroblast (3T3) using MTT assay. Fluorescence microscopy showed that GTN is able to induce apoptosis in HeLa cells in a time dependent manner. Flow cytometry further revealed HeLa cells treated with GTN to be arrested in the S phase. Phosphatidyl serine properties present during apoptosis enable early detection of the apoptosis in the cells. Using annexin V/PI double staining it could be shown that GTN induces early apoptosis on HeLa cells after 24, 48 and 72 h. It could be concluded that goniothalamin showing a promising cytotoxicity effect against several cancer cell lines including cervical cancer cells (HeLa) with apoptosis as the mode of cell death induced on HeLa cells by Goniothalamin was
format	Article
author	Manaf, Ali
author_facet	Manaf, Ali
author_sort	Manaf, Ali
title	Apoptosis induction, cell cycle arrest and in vitro anticancer activity of gonothalamin in a cancer cell lines
title_short	Apoptosis induction, cell cycle arrest and in vitro anticancer activity of gonothalamin in a cancer cell lines
title_full	Apoptosis induction, cell cycle arrest and in vitro anticancer activity of gonothalamin in a cancer cell lines
title_fullStr	Apoptosis induction, cell cycle arrest and in vitro anticancer activity of gonothalamin in a cancer cell lines
title_full_unstemmed	Apoptosis induction, cell cycle arrest and in vitro anticancer activity of gonothalamin in a cancer cell lines
title_sort	apoptosis induction, cell cycle arrest and in vitro anticancer activity of gonothalamin in a cancer cell lines
publishDate	2012
url	http://eprints.unisza.edu.my/2448/1/FH02-FPBSM-14-00629.pdf http://eprints.unisza.edu.my/2448/2/FH02-FPBSM-14-01142.jpg http://eprints.unisza.edu.my/2448/
_version_	1709673160457060352

Apoptosis induction, cell cycle arrest and in vitro anticancer activity of gonothalamin in a cancer cell lines

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