Whole genome sequencing of microbial pathogens: reading from the book of Acinetobacter (Malaysian Edition)
Acinetobacter baumannii is a Gram-negative nosocomial pathogen of concern due to its unique ability to acquire and develop resistance to all classes of antimicrobials. The World Health Organization (WHO) has even listed carbapenem-resistant A. baumannii as a top priority pathogen in urgent need o...
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| Main Author: | |
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| Format: | Conference or Workshop Item |
| Language: | English |
| Published: |
2021
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| Subjects: | |
| Online Access: | http://eprints.unisza.edu.my/4272/1/FH03-FP-21-55365.pdf http://eprints.unisza.edu.my/4272/ |
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| Institution: | Universiti Sultan Zainal Abidin |
| Language: | English |
| Summary: | Acinetobacter baumannii is a Gram-negative nosocomial pathogen of concern due to its unique
ability to acquire and develop resistance to all classes of antimicrobials. The World Health
Organization (WHO) has even listed carbapenem-resistant A. baumannii as a top priority
pathogen in urgent need of new antimicrobials. We have carried out whole genome sequencing
of A. baumannii along with non-baumannii Acinetobacter isolates obtained from the main
tertiary hospital in Terengganu, Malaysia, over a period of five years (2011 – 2016). Majority of
the Terengganu Acinetobacter hospital isolates were A. baumannii and WGS showed that the
multidrug resistant Global Clone 2 (GC2) lineage of A. baumannii was endemic in the
Terengganu hospital in 2011 – 2012 but that has been supplanted by a variety of other clones in
2015 – 2016. Our “Reading from the book of Acinetobacter” also led to the discovery of a large
ca. 170 kb potentially transmissible plasmid that encode the New Delhi metallo-β-lactamase-1
(NDM-1) and OXA-58 carbapenemase along with a host of other resistance genes in a clinical
isolate of A. baumannii and A. nosocomialis. Sequence analysis indicated that this plasmid was
derived from a cointegration of two separate plasmids that were reported from a clinical isolate
of A. pittii from Perak. The presence of such plasmids encoding resistance to multiple
antimicrobials warrants vigilance as its spread to susceptible strains would lead to increasing
incidence of antimicrobial resistance. |
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