Effect of pH, temperature and agitation on thermophilic biohydrogen production using immobilized cells on carbon composites (GAC-NiFe3O4)

• Main Authors: Engliman, Nurul Sakinah, Jamali, Nur Syakina
• Format: Article
• Language: English; English
• Published: Elsevier 2024
• Subjects: TP155 Chemical engineering
• Online Access: http://irep.iium.edu.my/112888/2/112888_Effect%20of%20pH%2C%20temperature%20and%20agitation%20on%20thermophilic%20biohydrogen.pdf; http://irep.iium.edu.my/112888/3/112888_Effect%20of%20pH%2C%20temperature%20and%20agitation%20on%20thermophilic%20biohydrogen_SCOPUS.pdf; http://irep.iium.edu.my/112888/; https://www.sciencedirect.com/science/article/abs/pii/S1385894724014669?via%3Dihub; https://doi.org/10.1016/j.cej.2024.149980
• Institution: Universiti Islam Antarabangsa Malaysia

Carbon composites-immobilized cell was utilized to enhance biohydrogen production. The initial pH, temperature and agitation effect was studied using the one-factor-at-a-time (OFAT) method. The optimal initial pH obtained was at pH 6.0 with hydrogen yield (HY) of 2.66 ± 0.09 mol H2/mol sugar, correlating with Gompertz constant of Hm = 974.99 mL, Rm = 17.90 mL/h and λ = 1.70 h. At 60 ◦C, the highest HY was obtained at 2.75 ± 0.11 mol H2/mol sugar. These corresponded to the Gompertz constant of Hm = 934.58 mL, Rm = 18.78 mL/h and λ = 1.89 h. The optimal agitation obtained was at 120 rpm, attaining the highest HY of 3.44 ± 0.54 mol H2/mol sugar, corresponding to the Gompertz constant of Hm = 1054.86 mL, Rm = 24.37 mL/h and λ = 3.90 h. The rRNA sequencing result revealed that the predominant species in the study was Thermoanaerobacterium. This study provides critical insight into process control conditions on biohydrogen production.