Development and application of a slot-blot assay using the damage sensing protein Atl1 to detect and quantify O6-Alkylated Guanine bases in DNA

• Main Authors: Yaakub, Hanum, Howell, Anthony, Margison, Geoffrey P, Povey, Andrew C
• Format: Article
• Language: English
• Published: Multidisciplinary Digital Publishing Institute (MDPI) 2024
• Subjects: RC254 Neoplasms. Tumors. Oncology (including Cancer); TP248.13 Biotechnology
• Online Access: http://irep.iium.edu.my/114314/1/114314_Development%20and%20application%20of%20a%20slot-blot%20assay.pdf; http://irep.iium.edu.my/114314/; https://www.mdpi.com/2305-6304/12/9/649
• Institution: Universiti Islam Antarabangsa Malaysia

Humans are unavoidably exposed to numerous different mutagenic DNA alkylating agents (AAs), but their role in the initiation of cancers is uncertain, in part due to difficulties in assessing human exposure. To address this, we have developed a screening method that measures promutagenic O6-alkylguan...