The effect of different hormones and incubation periods on in vitro proliferation of pineapple (ananas comosus I.) merr cv. smooth cayenne) shoot-tip culture

• Main Authors: Hamad, A.M., Taha, R.M.
• Format: Article
• Published: 2008
• Subjects: QH301 Biology
• Online Access: http://eprints.um.edu.my/10721/
• Institution: Universiti Malaya

Seven different hormone treatments, namely 6-benzylammopunne (BAP) at 2, 3 mg L-1 was applied singly and in combination with Indole Acetic Acid (IAA) at 0.18, 0.8 and 1.8 mg L-1, BAP at 3.3 mg L-1 in combination with IAA at 1.8 and 3.3 mg L-1 and triple combination of BAP at 2.3, IAA at 1.8 and Gibberellic acid (GA3) at 1.0 mg L -1 were tested, over four different incubation periods of 30, 45, 60 and 75 days, for their effect in the proliferation and growth of Smooth cayenne pineapple shoot-tip culture. Combined application of BAP at 3.3 and IAA at 1.8 mg L-1 induced the highest proliferation of 19 shoots/explant and the highest total of 121 and 125 shoots over 4 cycles of multiplication. Raising the IAA to 3.3 mg L-1 resulted in the lowest proliferation and stunted shoots. Incorporation of GA3 improved the shoot length but caused drastic reduction in proliferation. The other treatments showed an intermediate effect. © 2008 Asian Network for Scientific Information.