Antioxidant assay of Alstonia angustifolia ethanolic leaf extract

• Main Authors: Ab Rahim, Nurhidayah, Zakaria, Noorzafiza, Dzulkarnain, Syarifah Masyitah Habib, Azahar, Nazar Mohd Zabadi Mohd, Abdulla, Mahmood Ameen
• Format: Article
• Published: Penerbit Akademia Baru 2018
• Subjects: R Medicine
• Online Access: http://eprints.um.edu.my/22760/; http://www.akademiabaru.com/doc/ARFMTSV42_N1_P80_86.pdf
• Institution: Universiti Malaya

In current study, the ability of the ethanolic extract of Alstonia angustifolia in scavenging free radicals was assessed by using 1,1-diphenyl-2-picrylhydrazyl (DPPH), ferric reducing antioxidant power (FRAP), and hydrogen peroxide (H2O2) radical scavenging assay. The results suggested that the ethanolic extract of A. angustifolia leaves has a notable antioxidant activity. In FRAP assay, it showed that the extract have higher total antioxidant activity with FRAP value is 1868.33 μM/g Fe (ii) dry mass ± 0.15 than the control, quercetin with FRAP value is 1336.9 μM/g Fe (II) dry mass ± 0.12 and ascorbic acid with FRAP value is 1720 μM/g Fe (II) dry mass ± 0.02. For DPPH assay, the IC50 value of the extract is 384.77 while the IC50 value of standards of ascorbic acid and quercetin are 18.07 μg/ml and 39.60 μg/ml, respectively. For H2O2 scavenging assay, the IC50 value for the extract was discovered to be 186.77 μg/ml compared to standard ascorbic acid 466.56 μg/ml. Thus, the study suggests that A. angustifolia ethanolic leaf extract has a good origin of natural antioxidants and might be beneficial in impeding the oxidative stress progression thus averting diseases that related to free radicals.