Development of a Nested PCR for Environmental Detection of the Pathogenic free-living Amoeba Balamuthia mandrillaris
A DNA extraction and nested PCR method for detecting the pathogenic amoeba Balamuthia mandrillaris from the environment was developed. Sixteen of 17 Californian soil samples were positive compared with 0/44 from the United Kingdom. This approach will enable a greater understanding of B. mandrillaris...
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| Main Authors: | , , |
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| Format: | Article |
| Published: |
2011
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| Subjects: | |
| Online Access: | http://eprints.um.edu.my/4196/ |
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| Institution: | Universiti Malaya |
| Summary: | A DNA extraction and nested PCR method for detecting the pathogenic amoeba Balamuthia mandrillaris from the environment was developed. Sixteen of 17 Californian soil samples were positive compared with 0/44 from the United Kingdom. This approach will enable a greater understanding of B. mandrillaris ecology, geographic distribution, and public health risk. |
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