Development of a Nested PCR for Environmental Detection of the Pathogenic free-living Amoeba Balamuthia mandrillaris

Development of a Nested PCR for Environmental Detection of the Pathogenic free-living Amoeba Balamuthia mandrillaris

A DNA extraction and nested PCR method for detecting the pathogenic amoeba Balamuthia mandrillaris from the environment was developed. Sixteen of 17 Californian soil samples were positive compared with 0/44 from the United Kingdom. This approach will enable a greater understanding of B. mandrillaris...

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Main Authors: Ahmad, A.F., Andrew, P.W., Kilvington, S.
Format: Article
Published: 2011
Subjects:
R Medicine
Online Access:http://eprints.um.edu.my/4196/
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Institution: Universiti Malaya
id my.um.eprints.4196
record_format eprints
spelling my.um.eprints.41962012-12-13T02:00:48Z http://eprints.um.edu.my/4196/ Development of a Nested PCR for Environmental Detection of the Pathogenic free-living Amoeba Balamuthia mandrillaris Ahmad, A.F. Andrew, P.W. Kilvington, S. R Medicine A DNA extraction and nested PCR method for detecting the pathogenic amoeba Balamuthia mandrillaris from the environment was developed. Sixteen of 17 Californian soil samples were positive compared with 0/44 from the United Kingdom. This approach will enable a greater understanding of B. mandrillaris ecology, geographic distribution, and public health risk. 2011 Article PeerReviewed Ahmad, A.F. and Andrew, P.W. and Kilvington, S. (2011) Development of a Nested PCR for Environmental Detection of the Pathogenic free-living Amoeba Balamuthia mandrillaris. Journal of Eukaryotic Microbiology, 58 (3). pp. 269-271. ISSN 1066-5234 10.1111/j.1550-7408.2011.00541.x
institution Universiti Malaya
building UM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaya
content_source UM Research Repository
url_provider http://eprints.um.edu.my/
topic R Medicine
spellingShingle R Medicine
Ahmad, A.F.
Andrew, P.W.
Kilvington, S.
Development of a Nested PCR for Environmental Detection of the Pathogenic free-living Amoeba Balamuthia mandrillaris
description A DNA extraction and nested PCR method for detecting the pathogenic amoeba Balamuthia mandrillaris from the environment was developed. Sixteen of 17 Californian soil samples were positive compared with 0/44 from the United Kingdom. This approach will enable a greater understanding of B. mandrillaris ecology, geographic distribution, and public health risk.
format Article
author Ahmad, A.F.
Andrew, P.W.
Kilvington, S.
author_facet Ahmad, A.F.
Andrew, P.W.
Kilvington, S.
author_sort Ahmad, A.F.
title Development of a Nested PCR for Environmental Detection of the Pathogenic free-living Amoeba Balamuthia mandrillaris
title_short Development of a Nested PCR for Environmental Detection of the Pathogenic free-living Amoeba Balamuthia mandrillaris
title_full Development of a Nested PCR for Environmental Detection of the Pathogenic free-living Amoeba Balamuthia mandrillaris
title_fullStr Development of a Nested PCR for Environmental Detection of the Pathogenic free-living Amoeba Balamuthia mandrillaris
title_full_unstemmed Development of a Nested PCR for Environmental Detection of the Pathogenic free-living Amoeba Balamuthia mandrillaris
title_sort development of a nested pcr for environmental detection of the pathogenic free-living amoeba balamuthia mandrillaris
publishDate 2011
url http://eprints.um.edu.my/4196/
_version_ 1643687284845838336

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