Genetic diversity of the S-type small subunit ribosomal RNA gene of Plasmodium knowlesi isolates from Sabah, Malaysian Borneo and Peninsular Malaysia

Objective: To determine the genetic diversity of Plasmodium (P.) knowlesi isolates from Sabah, Malaysian Borneo and Peninsular Malaysia, targeting the S-type SSU rRNA gene and including aspects of natural selection and haplotype. Methods: Thirty-nine blood samples infected with P. knowlesi were coll...

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Main Authors: Chong, Eric Tzyy Jiann, Neoh, Joveen Wan Fen, Lau, Tiek Ying, Chua, Kek Heng, Lim, Yvonne Ai-Lian, Lee, Ping-Chin
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Published: Medknow Publications 2024
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Online Access:http://eprints.um.edu.my/45675/
https://doi.org/10.4103/apjtm.apjtm_540_23
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spelling my.um.eprints.456752024-11-07T07:00:51Z http://eprints.um.edu.my/45675/ Genetic diversity of the S-type small subunit ribosomal RNA gene of Plasmodium knowlesi isolates from Sabah, Malaysian Borneo and Peninsular Malaysia Chong, Eric Tzyy Jiann Neoh, Joveen Wan Fen Lau, Tiek Ying Chua, Kek Heng Lim, Yvonne Ai-Lian Lee, Ping-Chin RA Public aspects of medicine RA0421 Public health. Hygiene. Preventive Medicine Objective: To determine the genetic diversity of Plasmodium (P.) knowlesi isolates from Sabah, Malaysian Borneo and Peninsular Malaysia, targeting the S-type SSU rRNA gene and including aspects of natural selection and haplotype. Methods: Thirty-nine blood samples infected with P. knowlesi were collected in Sabah, Malaysian Borneo and Peninsular Malaysia. The S-type SSU rRNA gene was amplified using polymerase chain reaction, cloned into a vector, and sequenced. The natural selection and haplotype of the S-type SSU rRNA gene sequences were determined using DnaSP v6 and illustrated using NETWORK v10. This study's 39 S-type SSU rRNA sequences and eight sequences from the Genbank database were subjected to phylogenetic analysis using MEGA 11. Results: Overall, the phylogenetic analysis showed no evidence of a geographical cluster of P. knowlesi isolates from different areas in Malaysia based on the S-type SSU rRNA gene sequences. The S-type SSU rRNA gene sequences were relatively conserved and with a purifying effect. Haplotype sharing of the S-type SSU rRNA gene was observed between the P. knowlesi isolates in Sabah, Malaysian Borneo, but not between Sabah, Malaysian Borneo and Peninsular Malaysia. Conclusions: This study suggests that the S-type SSU rRNA gene of P. knowlesi isolates in Sabah, Malaysian Borneo, and Peninsular Malaysia has fewer polymorphic sites, representing the conservation of the gene. These features make the S-type SSU rRNA gene suitable for comparative studies, such as determining the evolutionary relationships and common ancestry among P. knowlesi species. Medknow Publications 2024-02 Article PeerReviewed Chong, Eric Tzyy Jiann and Neoh, Joveen Wan Fen and Lau, Tiek Ying and Chua, Kek Heng and Lim, Yvonne Ai-Lian and Lee, Ping-Chin (2024) Genetic diversity of the S-type small subunit ribosomal RNA gene of Plasmodium knowlesi isolates from Sabah, Malaysian Borneo and Peninsular Malaysia. Asian Pacific Journal of Tropical Medicine, 17 (2). pp. 84-90. ISSN 1995-7645, DOI https://doi.org/10.4103/apjtm.apjtm_540_23 <https://doi.org/10.4103/apjtm.apjtm_540_23>. https://doi.org/10.4103/apjtm.apjtm_540_23 10.4103/apjtm.apjtm_540_23
institution Universiti Malaya
building UM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaya
content_source UM Research Repository
url_provider http://eprints.um.edu.my/
topic RA Public aspects of medicine
RA0421 Public health. Hygiene. Preventive Medicine
spellingShingle RA Public aspects of medicine
RA0421 Public health. Hygiene. Preventive Medicine
Chong, Eric Tzyy Jiann
Neoh, Joveen Wan Fen
Lau, Tiek Ying
Chua, Kek Heng
Lim, Yvonne Ai-Lian
Lee, Ping-Chin
Genetic diversity of the S-type small subunit ribosomal RNA gene of Plasmodium knowlesi isolates from Sabah, Malaysian Borneo and Peninsular Malaysia
description Objective: To determine the genetic diversity of Plasmodium (P.) knowlesi isolates from Sabah, Malaysian Borneo and Peninsular Malaysia, targeting the S-type SSU rRNA gene and including aspects of natural selection and haplotype. Methods: Thirty-nine blood samples infected with P. knowlesi were collected in Sabah, Malaysian Borneo and Peninsular Malaysia. The S-type SSU rRNA gene was amplified using polymerase chain reaction, cloned into a vector, and sequenced. The natural selection and haplotype of the S-type SSU rRNA gene sequences were determined using DnaSP v6 and illustrated using NETWORK v10. This study's 39 S-type SSU rRNA sequences and eight sequences from the Genbank database were subjected to phylogenetic analysis using MEGA 11. Results: Overall, the phylogenetic analysis showed no evidence of a geographical cluster of P. knowlesi isolates from different areas in Malaysia based on the S-type SSU rRNA gene sequences. The S-type SSU rRNA gene sequences were relatively conserved and with a purifying effect. Haplotype sharing of the S-type SSU rRNA gene was observed between the P. knowlesi isolates in Sabah, Malaysian Borneo, but not between Sabah, Malaysian Borneo and Peninsular Malaysia. Conclusions: This study suggests that the S-type SSU rRNA gene of P. knowlesi isolates in Sabah, Malaysian Borneo, and Peninsular Malaysia has fewer polymorphic sites, representing the conservation of the gene. These features make the S-type SSU rRNA gene suitable for comparative studies, such as determining the evolutionary relationships and common ancestry among P. knowlesi species.
format Article
author Chong, Eric Tzyy Jiann
Neoh, Joveen Wan Fen
Lau, Tiek Ying
Chua, Kek Heng
Lim, Yvonne Ai-Lian
Lee, Ping-Chin
author_facet Chong, Eric Tzyy Jiann
Neoh, Joveen Wan Fen
Lau, Tiek Ying
Chua, Kek Heng
Lim, Yvonne Ai-Lian
Lee, Ping-Chin
author_sort Chong, Eric Tzyy Jiann
title Genetic diversity of the S-type small subunit ribosomal RNA gene of Plasmodium knowlesi isolates from Sabah, Malaysian Borneo and Peninsular Malaysia
title_short Genetic diversity of the S-type small subunit ribosomal RNA gene of Plasmodium knowlesi isolates from Sabah, Malaysian Borneo and Peninsular Malaysia
title_full Genetic diversity of the S-type small subunit ribosomal RNA gene of Plasmodium knowlesi isolates from Sabah, Malaysian Borneo and Peninsular Malaysia
title_fullStr Genetic diversity of the S-type small subunit ribosomal RNA gene of Plasmodium knowlesi isolates from Sabah, Malaysian Borneo and Peninsular Malaysia
title_full_unstemmed Genetic diversity of the S-type small subunit ribosomal RNA gene of Plasmodium knowlesi isolates from Sabah, Malaysian Borneo and Peninsular Malaysia
title_sort genetic diversity of the s-type small subunit ribosomal rna gene of plasmodium knowlesi isolates from sabah, malaysian borneo and peninsular malaysia
publisher Medknow Publications
publishDate 2024
url http://eprints.um.edu.my/45675/
https://doi.org/10.4103/apjtm.apjtm_540_23
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