Effect of Visible Light on Catalytic Hydrolysis of P-nitrophenyl Palmitate by the Pseudomonas Cepacia Lipase Immobilized on Sol–Gel Support

• Main Authors: Ponnarasy, Ganasen, Khan, Maksudur R., M., Abul Kalam, Mohd Sabri, Mahmud
• Format: Article
• Language: English; English; English
• Published: Springer Berlin Heidelberg 2014
• Subjects: TA Engineering (General). Civil engineering (General); TP Chemical technology
• Online Access: http://umpir.ump.edu.my/id/eprint/12375/1/Effect%20of%20visible%20light%20on%20catalytic%20hydrolysis%20.pdf; http://umpir.ump.edu.my/id/eprint/12375/2/Effect%20of%20visible%20light%20on%20catalytic%20hydrolysis.pdf; http://umpir.ump.edu.my/id/eprint/12375/13/Effect%20Of%20Visible%20Light%20On%20Catalytic%20Hydrolysis%20Of%20P-Nitrophenyl%20Palmitate%20By%20The%20Pseudomonas%20Cepacia%20Lipase%20Immobilized%20On%20Sol%E2%80%93Gel%20Support.pdf; http://umpir.ump.edu.my/id/eprint/12375/; http://dx.doi.org/10.1007/s00449-014-1213-6
• Institution: Universiti Malaysia Pahang

This paper demonstrates Pseudomonas cepacia lipase catalyzed hydrolysis of p-nitrophenyl palmitate under irradiation of light with wavelengths of 250–750 nm. The reaction follows Michaelis–Menten Kinetics and the light irradiation increases the overall rate of hydrolysis. Using Lineweaver–Burk plot K M and V max values for the reaction in presence of light are found to be 39.07 and 66.67 mM/min/g, respectively; while for the same reaction under dark condition, the values are 7.08 and 10.21 mM/min/g. The linear form of enzyme dependent rate of reaction confirms that no mass-transfer limitations are present and the reaction is a kinetically controlled enzymatic reaction.