Full Factorial Design for Production of Methyl 3-(3,5-Di-Tert-Butyl-4-Hydroxyphenyl) Propionate Using Oil Palm Frond Juice as a Sole Substrate by Ceratocystis fimbriata

• Main Authors: Nang Nor Azimah, Long Nadzri, Mior Ahmad Khushairi, Mohd Zahari, Saiful Nizam, Tajuddin, Che Mohd Aizal, Che Mohd
• Format: Article
• Language: English
• Published: Malaysian Society of Applied Biology 2018
• Subjects: TP Chemical technology
• Online Access: http://umpir.ump.edu.my/id/eprint/21772/1/Full%20Factorial%20Design%20for%20Production%20of%20Methyl%203.pdf; http://umpir.ump.edu.my/id/eprint/21772/; http://www.mabjournal.com/index.php?option=com_content&view=article&id=745&catid=59:current-view&Itemid=56
• Institution: Universiti Malaysia Pahang

Oil palm frond (OPF) juice has been known as a good source of sugars to replace commercial sugars to produce methyl 3- (3,5-di-tert-butyl-4-hydroxyphenyl) propionate. The aim of this study was to investigate the effect of temperature (°C), initial pH medium, agitation speed (rpm) and concentration of glucose (g/L) on the production of methyl 3-(3,5-di-tert-butyl-4-hydroxyphenyl) propionate from OPF juice by using Ceratocystis fimbriata (C. fimbriata). The design of experiment (DOE) method was used to screen the best parameters affecting the production of methyl 3-(3,5-di-tert-butyl-4-hydroxyphenyl) propionate. The Gas Chromatography-Mass Spectroscopy (GC-MS) with Solid Phase Micro Extraction (SPME) was used to analyze and separate the peak of methyl 3-(3,5-di-tert-butyl-4-hydroxyphenyl) propionate. Based on the GC-MS analysis, results showed that the most favorable condition for methyl 3-(3,5-di-tert-butyl-4-hydroxyphenyl) propionate production was achieved at an initial pH medium (8), agitation speed (100 rpm), temperature (25°C) and 30 g/L of glucose in OPF juice. Methyl 3-(3,5-di-tert-butyl-4-hydroxyphenyl) propionate were produced when the retention time was 32.80 min, the relative peak area was 0.24 % of chromatogram area. This result showed the great potential usage of OPF juice as a substrate to produce methyl 3-(3,5-di-tert-butyl-4-hydroxyphenyl) propionate by C. fimbriata.