Loop protein engineering for improved transglycosylation activity of a β‐N Acetylhexosaminidase

• Main Authors: Shariza, Jamek, Muschiol, Jan, Holck, Jesper, Zeuner, Birgitte, Busk, Peter K., Mikkelsen, Jørn D., Meyer, Anne S.
• Format: Article
• Language: English
• Published: John Wiley & Sons 2018
• Subjects: TP Chemical technology
• Online Access: http://umpir.ump.edu.my/id/eprint/22565/1/Loop%20protein%20engineering%20for%20improved%20transglycosylation%20activity.pdf; http://umpir.ump.edu.my/id/eprint/22565/; https://doi.org/10.1002/cbic.201800181
• Institution: Universiti Malaysia Pahang

Certain enzymes of the glycoside hydrolase family 20 (GH20) exert transglycosylation activity and catalyze the transfer of β‐N‐acetylglucosamine (GlcNAc) from a chitobiose donor to lactose to produce lacto‐N‐triose II (LNT2), a key human milk oligosaccharide backbone moiety. The present work is aime...