Chemical characterization of different types of herbs using instrumentation methods
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Universiti Malaysia Perlis
2011
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my.unimap-133492011-07-30T05:35:57Z Chemical characterization of different types of herbs using instrumentation methods Bidattul Syirat, Zainal Assoc. Prof. Dr Zainab Hamzah (Advisor) Herbs Herbal plant Orthosiphon stamineus Misai Kucing Catharanthus roseus Vinca rosea Kemunting Cina Anticancer drug Medicinal plant Access is limited to UniMAP community. A simple High Performance Liquid Chromatography (HPLC) and Fourier Transform Infrared (FTIR) methods were developed and validated for the determination of specific polyphenol components such as Rosmarinic Acid (RA), Eupatorin (EUP), Sinensitin (SIN) and 3’-hydroxy-5,6,7,4’-tetramethoxyflavone (TMF), as well as vincristine and vinblastine, in shoot, leave, stem and root extracts of Orthosiphon stamineus (also known as Misai Kucing) and Catharanthus roseus (also known as Vinca rosea) herb respectively. Separation and quantitative analysis of extracts using Ethanol Extraction, Water Extraction and Pressure Extraction were performed using Shimadzu HPLC System. An isocratic mobile phase consisted of water, methanol and tetrahydrofluoride (50:45:5, v/v) were used in this study. An FTIR analysis was performed to determine the functional groups present in the extracts. The concentrations of the phenolic compounds in the shoot, leave, stem and root extracts were determined using HPLC with ultraviolet detection at 340 nm. The analysis showed that the concentration of RA and EUP in leaves of Misai Kucing was higher than other parts of the plant. Meanwhile, the concentrations of vincristine and vinblastine in each parts of Vinca plant were undetectable. However, Water Extraction technique seems to be the most economical, time-consuming and environmental friendly compared to two other methods. 2011-07-30T05:35:57Z 2011-07-30T05:35:57Z 2011-05 Learning Object http://hdl.handle.net/123456789/13349 en Universiti Malaysia Perlis School of Bioprocess Engineering |
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Universiti Malaysia Perlis |
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Asia |
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Malaysia |
| content_provider |
Universiti Malaysia Perlis |
| content_source |
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http://dspace.unimap.edu.my/ |
| language |
English |
| topic |
Herbs Herbal plant Orthosiphon stamineus Misai Kucing Catharanthus roseus Vinca rosea Kemunting Cina Anticancer drug Medicinal plant |
| spellingShingle |
Herbs Herbal plant Orthosiphon stamineus Misai Kucing Catharanthus roseus Vinca rosea Kemunting Cina Anticancer drug Medicinal plant Bidattul Syirat, Zainal Chemical characterization of different types of herbs using instrumentation methods |
| description |
Access is limited to UniMAP community. |
| author2 |
Assoc. Prof. Dr Zainab Hamzah (Advisor) |
| author_facet |
Assoc. Prof. Dr Zainab Hamzah (Advisor) Bidattul Syirat, Zainal |
| format |
Learning Object |
| author |
Bidattul Syirat, Zainal |
| author_sort |
Bidattul Syirat, Zainal |
| title |
Chemical characterization of different types of herbs using instrumentation methods |
| title_short |
Chemical characterization of different types of herbs using instrumentation methods |
| title_full |
Chemical characterization of different types of herbs using instrumentation methods |
| title_fullStr |
Chemical characterization of different types of herbs using instrumentation methods |
| title_full_unstemmed |
Chemical characterization of different types of herbs using instrumentation methods |
| title_sort |
chemical characterization of different types of herbs using instrumentation methods |
| publisher |
Universiti Malaysia Perlis |
| publishDate |
2011 |
| url |
http://dspace.unimap.edu.my/xmlui/handle/123456789/13349 |
| _version_ |
1643790814825938944 |