Nanobioprobe for the determination of pork adulteration in burger formulations.
Link to publisher's homepage at http://www.hindawi.com/
Saved in:
| Main Authors: | , , , , |
|---|---|
| Other Authors: | |
| Format: | Article |
| Language: | English |
| Published: |
Hindawi Publishing Corporation
2013
|
| Subjects: | |
| Online Access: | http://dspace.unimap.edu.my/xmlui/handle/123456789/26006 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| Institution: | Universiti Malaysia Perlis |
| Language: | English |
| id |
my.unimap-26006 |
|---|---|
| record_format |
dspace |
| spelling |
my.unimap-260062014-04-08T08:57:27Z Nanobioprobe for the determination of pork adulteration in burger formulations. Md. Eaqub, Ali Shuhaimi, Mustafa, Assoc. Prof Dr. Uda, Hashim, Prof. Dr. Yaakob, Che Man, Prof. Dr Foo, Kai Loong uda@unimap.edu.my Meats Beef burger Nanobioprobe Pork adulteration Link to publisher's homepage at http://www.hindawi.com/ We report the development of a swine-specific hybrid nanobioprobe through a covalent integration of a fluorophore-labeled 27-nucleotide AluI-fragment of swine cytochrome b gene to a 3nm gold nanoparticle for the determination of pork adulteration in processed meat products. We tested the probe to estimate adulterated pork in ready-to-eat pork-spiked beef burgers. The probe quantitatively detected 1-100% spiked pork in burger formulations with 90% accuracy. A plot of observed fluorescence against the known concentration of AluI-digested pork DNA targets generated a concave curve, demonstrating a power relationship (y=2.956 x 0.509) with a regression coefficient (R 2) of 0.986. No cross-species detection was found in a standard set of pork, beef, chicken, mutton, and chevon burgers. The method is suitable for the determination of very short-length nucleic acid targets which cannot be estimated by conventional and real-time PCR but are essential for the determination of microRNA in biodiagnostics and degraded DNA in forensic testing and food analysis. 2013-06-24T04:23:53Z 2013-06-24T04:23:53Z 2012 Article Journal of Nanomaterials, vol. 2012, 2012, pages 1-7 1687-4110 http://www.hindawi.com/journals/jnm/2012/832387/ http://hdl.handle.net/123456789/26006 en Hindawi Publishing Corporation |
| institution |
Universiti Malaysia Perlis |
| building |
UniMAP Library |
| collection |
Institutional Repository |
| continent |
Asia |
| country |
Malaysia |
| content_provider |
Universiti Malaysia Perlis |
| content_source |
UniMAP Library Digital Repository |
| url_provider |
http://dspace.unimap.edu.my/ |
| language |
English |
| topic |
Meats Beef burger Nanobioprobe Pork adulteration |
| spellingShingle |
Meats Beef burger Nanobioprobe Pork adulteration Md. Eaqub, Ali Shuhaimi, Mustafa, Assoc. Prof Dr. Uda, Hashim, Prof. Dr. Yaakob, Che Man, Prof. Dr Foo, Kai Loong Nanobioprobe for the determination of pork adulteration in burger formulations. |
| description |
Link to publisher's homepage at http://www.hindawi.com/ |
| author2 |
uda@unimap.edu.my |
| author_facet |
uda@unimap.edu.my Md. Eaqub, Ali Shuhaimi, Mustafa, Assoc. Prof Dr. Uda, Hashim, Prof. Dr. Yaakob, Che Man, Prof. Dr Foo, Kai Loong |
| format |
Article |
| author |
Md. Eaqub, Ali Shuhaimi, Mustafa, Assoc. Prof Dr. Uda, Hashim, Prof. Dr. Yaakob, Che Man, Prof. Dr Foo, Kai Loong |
| author_sort |
Md. Eaqub, Ali |
| title |
Nanobioprobe for the determination of pork adulteration in burger formulations. |
| title_short |
Nanobioprobe for the determination of pork adulteration in burger formulations. |
| title_full |
Nanobioprobe for the determination of pork adulteration in burger formulations. |
| title_fullStr |
Nanobioprobe for the determination of pork adulteration in burger formulations. |
| title_full_unstemmed |
Nanobioprobe for the determination of pork adulteration in burger formulations. |
| title_sort |
nanobioprobe for the determination of pork adulteration in burger formulations. |
| publisher |
Hindawi Publishing Corporation |
| publishDate |
2013 |
| url |
http://dspace.unimap.edu.my/xmlui/handle/123456789/26006 |
| _version_ |
1643794807823269888 |