Optimization of chemical sterilization agent in Stevia rebaudiana tissue culture
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Universiti Malaysia Perlis (UniMAP)
2013
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| Online Access: | http://dspace.unimap.edu.my/xmlui/handle/123456789/27736 |
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my.unimap-277362013-08-27T08:41:40Z Optimization of chemical sterilization agent in Stevia rebaudiana tissue culture Hanna, Hashim Dr. Zarina Zakaria Tissue culture Stevia rebaudiana Chemical sterilization agent Sodium hypochloride (NaOCl) Access is limited to UniMAP community. This study was to distinguish the efficiency of using sodium hypochloride, (NaOCl) in sterilization of explant Stevia rebaudiana in tissue culture technique. Sterilization technique is ought to be done for the successful in growth of the tissue culture especially Stevia rebaudiana which is the type of plant to be studied. The tenacity of sterilization technique was to diminish the risk that the microorganisms sustain on the cultures. NaOCl is use as modification on using mercury chloride, (HgCl2) which was very harmful. The plant part used was leaf (1 cm) and culture in Murashige & Skoog media. By determining the optimal concentration and time immersed of the NaOCl to be used with existence of ethanol solution, the optimum solution attained was 0.70 % of NaOCl concentration with time immersed of 20 minutes and plus existence of ethanol. 2013-08-27T08:41:40Z 2013-08-27T08:41:40Z 2012-06 Learning Object http://hdl.handle.net/123456789/27736 en Universiti Malaysia Perlis (UniMAP) School of Bioprocess Engineering |
| institution |
Universiti Malaysia Perlis |
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UniMAP Library |
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Asia |
| country |
Malaysia |
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Universiti Malaysia Perlis |
| content_source |
UniMAP Library Digital Repository |
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http://dspace.unimap.edu.my/ |
| language |
English |
| topic |
Tissue culture Stevia rebaudiana Chemical sterilization agent Sodium hypochloride (NaOCl) |
| spellingShingle |
Tissue culture Stevia rebaudiana Chemical sterilization agent Sodium hypochloride (NaOCl) Hanna, Hashim Optimization of chemical sterilization agent in Stevia rebaudiana tissue culture |
| description |
Access is limited to UniMAP community. |
| author2 |
Dr. Zarina Zakaria |
| author_facet |
Dr. Zarina Zakaria Hanna, Hashim |
| format |
Learning Object |
| author |
Hanna, Hashim |
| author_sort |
Hanna, Hashim |
| title |
Optimization of chemical sterilization agent in Stevia rebaudiana tissue culture |
| title_short |
Optimization of chemical sterilization agent in Stevia rebaudiana tissue culture |
| title_full |
Optimization of chemical sterilization agent in Stevia rebaudiana tissue culture |
| title_fullStr |
Optimization of chemical sterilization agent in Stevia rebaudiana tissue culture |
| title_full_unstemmed |
Optimization of chemical sterilization agent in Stevia rebaudiana tissue culture |
| title_sort |
optimization of chemical sterilization agent in stevia rebaudiana tissue culture |
| publisher |
Universiti Malaysia Perlis (UniMAP) |
| publishDate |
2013 |
| url |
http://dspace.unimap.edu.my/xmlui/handle/123456789/27736 |
| _version_ |
1643795391517294592 |