Screening of vibro SPP from riverine environments using multiplex PCR
Monthly screening for the presence of Vibrio spp. from environmental sources in Kuching, Sarawak was done using the polymerase chain reaction (peR) technique. Nineteen samples were collected from different river sources each month during the six-month period (July-December, 2003). Multiplex peR wa...
Saved in:
| Main Author: | |
|---|---|
| Format: | Final Year Project Report |
| Language: | English |
| Published: |
Universiti Malaysia Sarawak, (UNIMAS)
2004
|
| Subjects: | |
| Online Access: | http://ir.unimas.my/id/eprint/19498/4/Lawrance%20full.pdf http://ir.unimas.my/id/eprint/19498/ |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| Institution: | Universiti Malaysia Sarawak |
| Language: | English |
| Summary: | Monthly screening for the presence of Vibrio spp. from environmental sources in Kuching, Sarawak was done
using the polymerase chain reaction (peR) technique. Nineteen samples were collected from different river
sources each month during the six-month period (July-December, 2003). Multiplex peR was used for
simultaneous detection of three major genus of Vibrio; Vibrio parahaemolylicus. Vibrio cholerae and Vibrio
vulnijicus in a single tube peR reaction. The three sets of primers II, ompWand vulCuisi were used to amplify
fragments of 450 bp, 588 bp and 222 bp in sizes for each species respectively. This application was useful for the
rapid detection of the Vibrio spp. from water sources. The results indicated that V parahaemolylicus was the
dominant species occurring about 61% through out the sampling period, followed by V cholerae (23%) and V
vulnijicus (16%). This study showed that Multiplex peR amplification was very specific, reduces labor and less
time consuming and is useful for diagnostic laboratories to detect the presence of Vibrio spp. and also for
epidemiological investigations. |
|---|