Enumeration of Escherichia coli O157:H7 in drinks from different food premises in Kota Samarahan

• Main Author: Nur Amiza Najihah, Ismail
• Format: Final Year Project Report
• Language: English; English
• Published: Universiti Malaysia Sarawak (UNIMAS) 2018
• Subjects: QR Microbiology
• Online Access: http://ir.unimas.my/id/eprint/35141/1/Nur%20Amiza.pdf; http://ir.unimas.my/id/eprint/35141/4/Nur%20Amiza%20full.pdf; http://ir.unimas.my/id/eprint/35141/
• Institution: Universiti Malaysia Sarawak

Contamination of drinks with E.coli O157:H7 served in food premises such as restaurants can cause haemorrhagic colitis and haemolytic uremic syndrome to humans. The presence or absence of faecal pathogen was demonstrated using coliform group as indicator microorganisms. Therefore, this study was conducted to detect the presence E.coli O157:H? in drinking water from restaurant to ensure safe and potable drinking water is served to the consumer. A total of 30 drinks samples including six samples of cold plain water, six samples of iced tea, and six. samples of iced milo, six samples of syrup and six samples of iced milk tea were bought from six restaurants in Kota Samarahan. Most Probable Number (MPN) procedure was used in this study to enumerate the MPN values of coliform bacteria in each drinks collected. The presence of stxl gene produced by E.coli O157:117 in water samples were identified by using PCR assay. There is one (3.33%) out of 30 samples were positive carried stxl gene. This study showed that one of the drinks collected from restaurants was contaminated with faecal contamination which was not safe to drink by the customers.