Association of Epstein-Barr Virus Latent Membrane Protein 1 (LMP1) Gene Expression and Caspase Activity in Normal Nasopharyngeal Cell
Objective: To assess LMP1 gene expression-induced apoptosis in normal nasopharyngeal cells by measuring caspase activity. Material and Methods: LMP1 gene was subcloned into pTracer and pcDNA vector, producing pTracer-LMP1 and pcDNA- LMP1 expression plasmids. The plasmids were then transfected into...
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| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
Japan University of Health Sciences & Japan International Cultural Exchange Foundation
2021
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| Subjects: | |
| Online Access: | http://ir.unimas.my/id/eprint/36350/1/membrane1.pdf http://ir.unimas.my/id/eprint/36350/ https://www.imj-1994.com/latest-issue/ |
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| Institution: | Universiti Malaysia Sarawak |
| Language: | English |
| Summary: | Objective: To assess LMP1 gene expression-induced apoptosis in normal nasopharyngeal cells by measuring caspase activity.
Material and Methods: LMP1 gene was subcloned into pTracer and pcDNA vector, producing pTracer-LMP1 and pcDNA-
LMP1 expression plasmids. The plasmids were then transfected into normal nasopharyngeal cells. LMP1 gene expression-induced
apoptosis was accessed by measuring Caspase activities using Caspase-Glo ® 3/7 Assay kit following the manufacturer's
protocol. The luminescence intensity was measured by microplate reader. Association of LMP1 gene expression with caspase
activation was analysed by independent Sample T test.
Results: LMP1 gene expression in normal nasopharyngeal cells is significantly associated with higher caspase activity of
apoptosis compare to the vector control with t(-2.142), p value of 0.03.
Conclusion: Our results show that, there is association of LMP1 gene expression and caspase activity level in normal nasopharyngeal
cell thus support that LMP1 gene expression is involved in apoptosis induction. |
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