Enterobacterial Repetitive Intragenic Consensus (ERIC) Genotyping of Escherichia coli o157 :H7
A total of 30 strains of Escherichia coli 0157:H7 isolated from beef and chicken burger were characterized by Enterobacterial Repetitive Intragenic Consensus(ERIC) genotyping. The ERIC polymorphism patterns obtained as illustrated in a dendrogram showed a significant discriminatory fingerprint among...
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| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Capricon Publication
2000
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| Subjects: | |
| Online Access: | http://ir.unimas.my/id/eprint/39297/2/Enterobacterial%20-%20Copy.PDF http://ir.unimas.my/id/eprint/39297/ https://scialert.net/abstract/?doi=pjbs.2000.35.37 |
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| Institution: | Universiti Malaysia Sarawak |
| Language: | English |
| Summary: | A total of 30 strains of Escherichia coli 0157:H7 isolated from beef and chicken burger were characterized by Enterobacterial Repetitive Intragenic Consensus(ERIC) genotyping. The ERIC polymorphism patterns obtained as illustrated in a dendrogram showed a significant discriminatory fingerprint among the 30 E. coli O157:H7 strains. Nearly every isolates had a unique fingerprint and that there were no bands that were highly conserved among the isolates. This study suggests that there is considerable genetic heterogeneity among the E. coli O157:H7 strains by ERIC PCR, and that this has application in screening strains from clinical or food samples to detect a virulent strain with a known fingerprint, and to trace its dissemination. |
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