Molecular Characterization and Similarity Relationships Among Selected Kelampayan (Neolamarchia cadamba) Genotypes from Kelampayan Provenance Trial Plot I Using ISSR Markers

• Main Author: Tiong, Shing Yiing
• Format: Final Year Project Report
• Language: English
• Published: Universiti Malaysia Sarawak, UNIMAS 2010
• Subjects: SB Plant culture
• Online Access: http://ir.unimas.my/id/eprint/7877/4/Shing%20Yiing%28fulltext%29.pdf; http://ir.unimas.my/id/eprint/7877/
• Institution: Universiti Malaysia Sarawak

Neolamarckia cadamba or Kelampayan is a fast growing timber species that has economical and medicinal value. Kelampayan is one of the species widely used for reforestation programme, so as high commercial value in plywood manufacturing and wound cleansing. Therefore, molecular characterization of this species is important to enable further species improvement. In this project, determination of genetic relatedness and diversity of kelampayan was accomplish using ISSR primers namely (GTG)6, (AC)10 and (AG)10. Selected primers had successful amplified 64 loci with 73.44% of polymorphic in block I population. Shannon’s diversity index of all three combined primes was reported to be 0.350. Molecular diversity based on Jaccard’s similarity coefficient of 79 trees was in the ranged of 0.14 to 0.94. UPGMA dendrograms and neighbor joining tree were also constructed to show the genetic relationship of N. cadamba trees. In the outcome of the dendrograms reveals that, a total of four clusters were grouped for block 1 kelampayan trees indicating that N. cadamba trees are genetically diverse.