Knotting terminal ends of mutant T1 lipase with disulfide bond improved structure rigidity and stability

• Main Authors: Hamdan, Siti Hajar, Maiangwa, Jonathan, Nezhad, Nima Ghahremani, Mohamad Ali, Mohd Shukuri, M. Yahaya, Normi, Mohd Shariff, Fairolniza, Raja Abd Rahman, Raja Noor Zaliha, Leow, Thean Chor
• Format: Article
• Published: Springer 2023
• Online Access: http://psasir.upm.edu.my/id/eprint/109091/; https://link.springer.com/article/10.1007/s00253-023-12396-5?error=cookies_not_supported&code=3a9c2fcb-c8f7-45c4-9e6e-868100a78c27
• Institution: Universiti Putra Malaysia

Lipase biocatalysts offer unique properties which are often impaired by low thermal and methanol stability. In this study, the rational design was employed to engineer a disulfide bond in the protein structure of Geobacillus zalihae T1 lipase in order to improve its stability. The selection of targe...