Production of mannan-degrading enzymes from Aspergillus niger and Sclerotium rolfsii using palm kernel cake as carbon source.
The aim of this study is to produce and profile the mannan-degrading enzymes from local fungal isolates by submerged fermentation and saccharification of PKC. Results showed that Aspergillus niger and Sclerotium rolfsii can produce mannan-degrading enzymes. The fungi were grown in submerged fermenta...
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| Main Authors: | , , , |
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| Format: | Article |
| Language: | English English |
| Published: |
Academic Journals Inc.
2009
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| Online Access: | http://psasir.upm.edu.my/id/eprint/16360/1/Production%20of%20mannan.pdf http://psasir.upm.edu.my/id/eprint/16360/ |
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| Institution: | Universiti Putra Malaysia |
| Language: | English English |
| Summary: | The aim of this study is to produce and profile the mannan-degrading enzymes from local fungal isolates by submerged fermentation and saccharification of PKC. Results showed that Aspergillus niger and Sclerotium rolfsii can produce mannan-degrading enzymes. The fungi were grown in submerged fermentation of PKC to produce mannan-degrading enzymes. The highest alpha-galactosidase was obtained on day 13 of fermentation (0.128 U mL-1) when using A. niger and on day 18 (0.126 U mL-1) when using S. rolfsii. Analysis also showed that enzyme activities for beta-mannanase using S. rolfsii were the highest at day 17 (3.166 U mL-1) and for A. niger (2.482 U mL-1) at day 8. Meanwhile the highest beta-mannosidase were obtained at day 16 for A. niger (0.128 U mL-1) and for S. rolfsii at day 16 (0.116 U mL-1). |
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