Production of mannan-degrading enzymes from Aspergillus niger and Sclerotium rolfsii using palm kernel cake as carbon source.

Production of mannan-degrading enzymes from Aspergillus niger and Sclerotium rolfsii using palm kernel cake as carbon source.

The aim of this study is to produce and profile the mannan-degrading enzymes from local fungal isolates by submerged fermentation and saccharification of PKC. Results showed that Aspergillus niger and Sclerotium rolfsii can produce mannan-degrading enzymes. The fungi were grown in submerged fermenta...

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Main Authors: Abd. Aziz, Suraini, Ab Razak, Nor Asma, Musa, Mohamed Hanafi, Hassan, Mohd Ali
Format: Article
Language:English
English
Published: Academic Journals Inc. 2009
Online Access:http://psasir.upm.edu.my/id/eprint/16360/1/Production%20of%20mannan.pdf
http://psasir.upm.edu.my/id/eprint/16360/
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Institution: Universiti Putra Malaysia
Language: English
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spelling my.upm.eprints.163602015-11-26T07:05:42Z http://psasir.upm.edu.my/id/eprint/16360/ Production of mannan-degrading enzymes from Aspergillus niger and Sclerotium rolfsii using palm kernel cake as carbon source. Abd. Aziz, Suraini Ab Razak, Nor Asma Musa, Mohamed Hanafi Hassan, Mohd Ali The aim of this study is to produce and profile the mannan-degrading enzymes from local fungal isolates by submerged fermentation and saccharification of PKC. Results showed that Aspergillus niger and Sclerotium rolfsii can produce mannan-degrading enzymes. The fungi were grown in submerged fermentation of PKC to produce mannan-degrading enzymes. The highest alpha-galactosidase was obtained on day 13 of fermentation (0.128 U mL-1) when using A. niger and on day 18 (0.126 U mL-1) when using S. rolfsii. Analysis also showed that enzyme activities for beta-mannanase using S. rolfsii were the highest at day 17 (3.166 U mL-1) and for A. niger (2.482 U mL-1) at day 8. Meanwhile the highest beta-mannosidase were obtained at day 16 for A. niger (0.128 U mL-1) and for S. rolfsii at day 16 (0.116 U mL-1). Academic Journals Inc. 2009 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/16360/1/Production%20of%20mannan.pdf Abd. Aziz, Suraini and Ab Razak, Nor Asma and Musa, Mohamed Hanafi and Hassan, Mohd Ali (2009) Production of mannan-degrading enzymes from Aspergillus niger and Sclerotium rolfsii using palm kernel cake as carbon source. Research Journal of Agriculture and Biological Sciences, 3 (2). pp. 251-256. ISSN 1819-3412 10.3923/rjes.2009.251.256 English
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
English
description The aim of this study is to produce and profile the mannan-degrading enzymes from local fungal isolates by submerged fermentation and saccharification of PKC. Results showed that Aspergillus niger and Sclerotium rolfsii can produce mannan-degrading enzymes. The fungi were grown in submerged fermentation of PKC to produce mannan-degrading enzymes. The highest alpha-galactosidase was obtained on day 13 of fermentation (0.128 U mL-1) when using A. niger and on day 18 (0.126 U mL-1) when using S. rolfsii. Analysis also showed that enzyme activities for beta-mannanase using S. rolfsii were the highest at day 17 (3.166 U mL-1) and for A. niger (2.482 U mL-1) at day 8. Meanwhile the highest beta-mannosidase were obtained at day 16 for A. niger (0.128 U mL-1) and for S. rolfsii at day 16 (0.116 U mL-1).
format Article
author Abd. Aziz, Suraini
Ab Razak, Nor Asma
Musa, Mohamed Hanafi
Hassan, Mohd Ali
spellingShingle Abd. Aziz, Suraini
Ab Razak, Nor Asma
Musa, Mohamed Hanafi
Hassan, Mohd Ali
Production of mannan-degrading enzymes from Aspergillus niger and Sclerotium rolfsii using palm kernel cake as carbon source.
author_facet Abd. Aziz, Suraini
Ab Razak, Nor Asma
Musa, Mohamed Hanafi
Hassan, Mohd Ali
author_sort Abd. Aziz, Suraini
title Production of mannan-degrading enzymes from Aspergillus niger and Sclerotium rolfsii using palm kernel cake as carbon source.
title_short Production of mannan-degrading enzymes from Aspergillus niger and Sclerotium rolfsii using palm kernel cake as carbon source.
title_full Production of mannan-degrading enzymes from Aspergillus niger and Sclerotium rolfsii using palm kernel cake as carbon source.
title_fullStr Production of mannan-degrading enzymes from Aspergillus niger and Sclerotium rolfsii using palm kernel cake as carbon source.
title_full_unstemmed Production of mannan-degrading enzymes from Aspergillus niger and Sclerotium rolfsii using palm kernel cake as carbon source.
title_sort production of mannan-degrading enzymes from aspergillus niger and sclerotium rolfsii using palm kernel cake as carbon source.
publisher Academic Journals Inc.
publishDate 2009
url http://psasir.upm.edu.my/id/eprint/16360/1/Production%20of%20mannan.pdf
http://psasir.upm.edu.my/id/eprint/16360/
_version_ 1643826191089532928

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