Quantification of infectious recombinant murine MSCV-based retroviruses using PCR approach

• Main Authors: Nik Abd Rahman, Nik Mohd Afizan, Allaudin, Zeenathul Nazariah, Ismail, Ruzila, Mustafa, Nor Hidayah, Mohd Lila, Mohd Azmi
• Format: Article
• Language: English
• Published: EuroJournals Publishing 2010
• Online Access: http://psasir.upm.edu.my/id/eprint/16410/1/Quantification%20of%20infectious%20recombinant%20murine%20MSCV.pdf; http://psasir.upm.edu.my/id/eprint/16410/
• Institution: Universiti Putra Malaysia

The current available molecular method to detect the retroviral-mediated gene expression is reverse transcriptase-polymerase chain reaction (RT-PCR). However, the conventional PCR is time consuming, prone to error and not sensitive. In this paper, we performed a real-time PCR assay to quantify retro...