Virus-like particles of Macrobrachium rosenbergii nodavirus produced in bacteria

• Main Authors: Zee, Hong Goh, Tan, Soon Guan, Bhassu, Subha, Tan, Wen Siang
• Format: Article
• Language: English
• Published: Elsevier 2011
• Online Access: http://psasir.upm.edu.my/id/eprint/22325/1/Virus-like%20particles%20of%20Macrobrachium%20rosenbergii%20nodavirus%20produced%20in%20bacteria.pdf; http://psasir.upm.edu.my/id/eprint/22325/
• Institution: Universiti Putra Malaysia

Macrobrachium rosenbergii nodavirus (MrNv) infects giant freshwater prawns and causes white tail disease (WTD). The coding region of the capsid protein of MrNv was amplified with RT-PCR and cloned into the pTrcHis2-TOPO vector. The recombinant plasmid was introduced into Escherichia coli and protein expression was induced with IPTG. SDS-PAGE showed that the recombinant protein containing the His-tag and myc epitope has a molecular mass of about 46kDa and it was detected by the anti-His antibody in Western blotting. The protein was purified using immobilized metal affinity chromatography (IMAC) and transmission electron microscopic analysis revealed that the recombinant protein assembled into VLPs.