Native agarose gel electrophoresis and electroelution: a fast and cost-effective method to separate the small and large hepatitis B capsids

• Main Authors: Yoon, Kam Yee, Tan, Wen Siang, Tey, Beng Ti, Lee, Khai Wooi, Ho, Kok Lian
• Format: Article
• Published: Wiley-VCH Verlag 2013
• Online Access: http://psasir.upm.edu.my/id/eprint/29546/; http://onlinelibrary.wiley.com/doi/10.1002/elps.v34.2/issuetoc
• Institution: Universiti Putra Malaysia

Hepatitis B core antigen (HBcAg) expressed in Escherichia coli is able to self-assemble into large and small capsids comprising 240 (triangulation number T = 4) and 180 (triangulation number T = 3) subunits, respectively. Conventionally, sucrose density gradient ultracentrifugation and SEC have been...