Isolation, subcellular localization and phylogenetic of transaldolase genes from Zea mays cv sweet corn bi-color

• Main Authors: Kalhori, Nahid, Nulit, Rosimah, Ang, Poh Nee
• Format: Conference or Workshop Item
• Language: English
• Published: Department of Biology, Faculty of Science, Universiti Putra Malaysia 2014
• Online Access: http://psasir.upm.edu.my/id/eprint/31298/1/BIO%202014-66.pdf; http://psasir.upm.edu.my/id/eprint/31298/
• Institution: Universiti Putra Malaysia

The Pentose Phosphate Pathway (PPP) also is known as 6-phosphogluconate pathway which occurs in the cytosol of the plant cell. There are two main arms in PPP; the oxidative and non-oxidative arms. The main functions of this pathway is to generate reducing equivalents in the form of NADPH, for reductive biosynthesis reactions within cells in the biosynthesis of fatty acids and steroid. This pathway also provide the cell with ribose-5-phosphate (R5P) for the synthesis of the nucleotides and nucleic acids. Transaldolase (TAL) is an enzyme which plays an important role in the non-oxidative portion of the pentose phosphate pathway. The actual reaction is between glyceraldehydes 3-phosphate and sedoheptulose 7-phosphate, resulting the formation of C4 product erythrose 4-phosphate and fructose 6-phosphate. In this study, transaldolase (TAL) has been successfully isolated and identified from Zea mays cv Sweet corn bi-color. The objectives of the study were achieved where the specific primer designed has function effectively in isolated TAL with 773 bp of nucleotide sequences. Analysis of homology through multiple sequence alignment revealed that TAL from Z. mays cv Sweet corn bi-color is highly similar with the plant species compared to animal and bacteria. ClustalW analysis found that TAL from Z. mays hit the score of 85.0 as they are close related in terms of taxonomy level which they are from the family of Poaceae. Other plants such as Solanum lycopersicum and Solanum tuberosum are from family Solanaceae, Dimorcarpus longan is from family Sapindaceae whereas Hyacinthus orientalis is from Hyacinthus family. However, it is discovered that TAL from Z. mays cv Sweet corn bi-color with TAL in Oryza sativa subsp. Indica only possess score of 64.0.