Molecular characterization of a recombinant managanese superoxide dismutase from Lactococcus lactis M4

• Main Authors: Tan, Boon Hooi, Leow, Adam Thean Chor, Foo, Hooi Ling, Abdul Rahim, Raha
• Format: Article
• Language: English
• Published: Hindawi Publishing Corporation 2014
• Online Access: http://psasir.upm.edu.my/id/eprint/34572/1/Molecular%20Characterization%20of%20a%20Recombinant%20Manganese.pdf; http://psasir.upm.edu.my/id/eprint/34572/; http://www.hindawi.com/journals/bmri/2014/469298/abs/
• Institution: Universiti Putra Malaysia

A superoxide dismutase (SOD) gene of Lactococcus lactis M4 was cloned and expressed in a prokaryotic system. Sequence analysis revealed an open reading frame of 621 bp which codes for 206 amino acid residues. Expression of sodA under T7 promoter exhibited a specific activity of 4967 U/mg when induce...