Pichia pastoris as a host to overexpress the thermostable T1 lipase from Geobacillus zalihae

• Main Authors: Oslan, Siti Nurbaya, Salleh, Abu Bakar, Raja Abdul Rahman, Raja Noor Zaliha, Leow, Adam Thean Chor, Basri, Mahiran
• Format: Article
• Language: English
• Published: Global Science and Technology Forum 2014
• Online Access: http://psasir.upm.edu.my/id/eprint/34942/1/Pichia%20pastoris%20as%20a%20host%20to%20overexpress%20the%20thermostable%20T1%20lipase%20from%20Geobacillus%20zalihae.pdf; http://psasir.upm.edu.my/id/eprint/34942/; http://dl6.globalstf.org/index.php/jbio/article/view/1002
• Institution: Universiti Putra Malaysia

Pichia pastoris was known to be a good expression system in producing various heterologous proteins. The gene encoding thermostable Tl lipase from Geobacillus zalihae was cloned into pPICZαB and expressed in P. pastoris strains (GS115, X-33 and KM71H) under regulation of alcohol oxidase promoter. T...