Development of highly sensitive immunosensor for clenbuterol detection by using poly(3,4-ethylenedioxythiophene)/graphene oxide modified screen-printed carbon electrode

• Main Authors: A. Talib, Nurul Ain, Salam, Faridah, Sulaiman, Yusran
• Format: Article
• Language: English
• Published: MDPI 2018
• Online Access: http://psasir.upm.edu.my/id/eprint/38098/1/38098.pdf; http://psasir.upm.edu.my/id/eprint/38098/; https://www.mdpi.com/1424-8220/18/12/4324
• Institution: Universiti Putra Malaysia

Clenbuterol (CLB) is an antibiotic and illegal growth promoter drug that has a long half-life and easily remains as residue and contaminates the animal-based food product that leads to various health problems. In this work, electrochemical immunosensor based on poly(3,4-ethylenedioxythiophene)/graphene oxide (PEDOT/GO) modified screen-printed carbon electrode (SPCE) for CLB detection was developed for antibiotic monitoring in a food product. The modification of SPCE with PEDOT/GO as a sensor platform was performed through electropolymerization, while the electrochemical assay was accomplished while using direct competitive format in which the free CLB and clenbuterol-horseradish peroxidase (CLB-HRP) in the solution will compete to form binding with the polyclonal anti-clenbuterol antibody (Ab) immobilized onto the modified electrode surface. A linear standard CLB calibration curve with R2 = 0.9619 and low limit of detection (0.196 ng mL−1) was reported. Analysis of milk samples indicated that this immunosensor was able to detect CLB in real samples and the results that were obtained were comparable with enzyme-linked immunosorbent assays (ELISA).