Production of phytase by Mitsuokella jalaludinii in semi-solid state fermentation of agricultural by-products

• Main Authors: Tang, Hooi Chia, Sieo, Chin Chin, Abdullah, Norhani, Mohamad, Rosfarizan, Omar, Abdul Rahman, Chong, Chun Wie, Fitrianto, Anwar, Ho, Yin Wan
• Format: Article
• Language: English
• Published: Penerbit Universiti Kebangsaan Malaysia 2018
• Online Access: http://psasir.upm.edu.my/id/eprint/60473/1/Production%20of%20phytase%20by%20Mitsuokella%20jalaludinii%20in%20semi-solid%20state%20fermentation%20of%20agricultural%20by-products.pdf; http://psasir.upm.edu.my/id/eprint/60473/; http://www.ukm.my/jsm/english_journals/vol47num2_2018/contentsVol47num2_2018.html
• Institution: Universiti Putra Malaysia

Phytase activity and growth of anaerobic rumen bacterium, Mitsuokella jalaludinii were investigated by semi-solid state fermentation. Carbon source (rice bran, yam and cassava), nitrogen sources (soya bean, offal meal, fish meal and feather meal) and growth factors (hemin, L-cysteine hydrochloride and minerals) were evaluated in a one-factor-at-a-time approach. Rice bran and fish meal produced better growth and phytase enzyme activity. The removal of L-cysteine hydrochloride and minerals significantly decreased (p<0.05) phytase activity from 1178.72 U to 446.99 U and 902.54 U, respectively. The response surface methods (RSM) was conducted to optimize the phytase production and the results showed the combination of 7.7% of rice bran and 3.7% of fish meal in semi-solid state fermentation gave the highest phytase activity. Maximum phytase production and optimum growth of bacteria were detected at 12 h incubation in both MF medium (control) and agro-medium. In this agro-medium, M. jalaludinii produced 2.5 fold higher phytase activity compared to MF medium.