Development of bacteriophage ΦKAZ14 loaded chitosan nanoparticles for biological control of colibacillosis in chickens

Bacteriophage ΦKAZ14 was applied in the biological control of colibacillosis. It had lytic activity on APEC 01: K1:H7 and β lactamase producing Escherichia coli. The icosahedral head of the phage measured 50 nm by 45 nm and long contractile tail of 78 nm by 10 nm. It multiplicity of infection (MOI)...

Full description

Saved in:
Bibliographic Details
Main Author: Kaikabo, Adamu Ahmad
Format: Thesis
Language:English
Published: 2016
Online Access:http://psasir.upm.edu.my/id/eprint/69412/1/FSTM%202016%202%20IR.pdf
http://psasir.upm.edu.my/id/eprint/69412/
Tags: Add Tag
No Tags, Be the first to tag this record!
Institution: Universiti Putra Malaysia
Language: English
id my.upm.eprints.69412
record_format eprints
spelling my.upm.eprints.694122019-07-01T08:11:57Z http://psasir.upm.edu.my/id/eprint/69412/ Development of bacteriophage ΦKAZ14 loaded chitosan nanoparticles for biological control of colibacillosis in chickens Kaikabo, Adamu Ahmad Bacteriophage ΦKAZ14 was applied in the biological control of colibacillosis. It had lytic activity on APEC 01: K1:H7 and β lactamase producing Escherichia coli. The icosahedral head of the phage measured 50 nm by 45 nm and long contractile tail of 78 nm by 10 nm. It multiplicity of infection (MOI) was 0.01, the latent period of infection of 15 minutes and burst size of 80 particles per infected cell. The phage survived a pH range of 5.0 to 14, and resistant to a temperature of 65 °C. C-NPs protected the phage from enzymatic degradation in vitro. The spherical C-NPs had average particle size range from 176 ± 3.2 to 188 ± 7.4 nm, a zeta potential of 60.3±0.2 and 50.5±0.4 and polydispersity index (PDI) of 0.506 and 0.472. Fourier transform infrared spectroscopy (FTIR) showed no difference between C-ΦKAZ14 NP and C-NP. Thermal analysis showed the temperature of -20 °C is desirable for storage. C-ΦKAZ14 NPs were not toxic on chorioallantoic membrane. Treatment with C- ΦKAZ14 NPs decrease viable bacterial counts in feces and colonization in the intestine of chickens (p ≤ 0.05). It also significantly decreased mortality rate in E. coli-challenged birds (p ≤ 0.05). C- ΦKAZ14 NPs increased bodyweight of chickens (p ≤ 0.05). Lesions were not observed in major organs. C- ΦKAZ14 NPs therapy is a beneficial control strategy against colibacillosis in chickens. 2016-06 Thesis NonPeerReviewed text en http://psasir.upm.edu.my/id/eprint/69412/1/FSTM%202016%202%20IR.pdf Kaikabo, Adamu Ahmad (2016) Development of bacteriophage ΦKAZ14 loaded chitosan nanoparticles for biological control of colibacillosis in chickens. PhD thesis, Universiti Putra Malaysia.
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description Bacteriophage ΦKAZ14 was applied in the biological control of colibacillosis. It had lytic activity on APEC 01: K1:H7 and β lactamase producing Escherichia coli. The icosahedral head of the phage measured 50 nm by 45 nm and long contractile tail of 78 nm by 10 nm. It multiplicity of infection (MOI) was 0.01, the latent period of infection of 15 minutes and burst size of 80 particles per infected cell. The phage survived a pH range of 5.0 to 14, and resistant to a temperature of 65 °C. C-NPs protected the phage from enzymatic degradation in vitro. The spherical C-NPs had average particle size range from 176 ± 3.2 to 188 ± 7.4 nm, a zeta potential of 60.3±0.2 and 50.5±0.4 and polydispersity index (PDI) of 0.506 and 0.472. Fourier transform infrared spectroscopy (FTIR) showed no difference between C-ΦKAZ14 NP and C-NP. Thermal analysis showed the temperature of -20 °C is desirable for storage. C-ΦKAZ14 NPs were not toxic on chorioallantoic membrane. Treatment with C- ΦKAZ14 NPs decrease viable bacterial counts in feces and colonization in the intestine of chickens (p ≤ 0.05). It also significantly decreased mortality rate in E. coli-challenged birds (p ≤ 0.05). C- ΦKAZ14 NPs increased bodyweight of chickens (p ≤ 0.05). Lesions were not observed in major organs. C- ΦKAZ14 NPs therapy is a beneficial control strategy against colibacillosis in chickens.
format Thesis
author Kaikabo, Adamu Ahmad
spellingShingle Kaikabo, Adamu Ahmad
Development of bacteriophage ΦKAZ14 loaded chitosan nanoparticles for biological control of colibacillosis in chickens
author_facet Kaikabo, Adamu Ahmad
author_sort Kaikabo, Adamu Ahmad
title Development of bacteriophage ΦKAZ14 loaded chitosan nanoparticles for biological control of colibacillosis in chickens
title_short Development of bacteriophage ΦKAZ14 loaded chitosan nanoparticles for biological control of colibacillosis in chickens
title_full Development of bacteriophage ΦKAZ14 loaded chitosan nanoparticles for biological control of colibacillosis in chickens
title_fullStr Development of bacteriophage ΦKAZ14 loaded chitosan nanoparticles for biological control of colibacillosis in chickens
title_full_unstemmed Development of bacteriophage ΦKAZ14 loaded chitosan nanoparticles for biological control of colibacillosis in chickens
title_sort development of bacteriophage φkaz14 loaded chitosan nanoparticles for biological control of colibacillosis in chickens
publishDate 2016
url http://psasir.upm.edu.my/id/eprint/69412/1/FSTM%202016%202%20IR.pdf
http://psasir.upm.edu.my/id/eprint/69412/
_version_ 1643839486709202944