Production of L2 lipase by Bacillus sp. strain L2: nutritional and physical factors

• Main Authors: Mohd Shariff, Fairolniza, Leow, Thean Chor, Mukred, Abdul Daim, Salleh, Abu Bakar, Basri, Mahiran, Raja Abdul Rahman, Raja Noor Zaliha
• Format: Article
• Language: English
• Published: Wiley-VCH Verlag GmbH & Co. KGaA 2007
• Online Access: http://psasir.upm.edu.my/id/eprint/7508/1/Production%20of%20L2%20lipase%20by%20Bacillus%20sp.pdf; http://psasir.upm.edu.my/id/eprint/7508/; http://onlinelibrary.wiley.com/wol1/doi/10.1002/jobm.200610275/abstract
• Institution: Universiti Putra Malaysia

A thermophilic bacterium, Bacillus sp. strain L2 was isolated from a hot spring in Perak, Malaysia. An extracellular lipase activity was detected through plate and broth assays at 70 °C after 28 h of incubation. The L2 lipase production was growth dependent as revealed by a number of factors affecting the secretion of extracelullar lipase. As for nutritional factors, casamino acids, trehalose, Ca2+ and Tween 60 were found to be more effective for lipase production. The optimum physical condition for L2 lipase production was obtained at 70 °C after 28 h of cultivation time, at pH 7.0, 150 rpm of agitation rate and 1% of starting inoculum size. The activity staining of crude L2 lipase revealed a clearing zone at 39 kDa.