Application of Plackett Burman design for antioxidant extraction from Actinodaphne sesquipedalis leaves

• Main Authors: Jamaludin, Siti Fatihah Balqis, Abd Gani, Siti Salwa, Zaidan, Uswatun Hasanah, Bawon, Paiman
• Format: Article
• Language: English
• Published: Blue Eyes Intelligence Engineering & Sciences Publication 2019
• Online Access: http://psasir.upm.edu.my/id/eprint/80706/1/PLACKETT.pdf; http://psasir.upm.edu.my/id/eprint/80706/; https://www.ijeat.org/wp-content/uploads/papers/v8i6S3/F10080986S319.pdf
• Institution: Universiti Putra Malaysia

Actinodaphne sesquipedalis Hook. F. Var. Glabra (Kochummen), also known as “Medang payung” by the Malay people, belongs to the Lauraceae family. In this study, a Plackett-Burman design was used to evaluate the significant extraction parameters in achieving maximum DPPH radical scavenging activity from ethanol leaves extract of A. sesquipedalis. Microwave-assisted extraction technique was employed using aqueous ethanol. The independent parameters were microwave power level (30–60 W), feed-to-solvent ratio (1:30 g/ml), irradiation time (30-90 s) and ethanol concentration (20–80%). Amongst the examined parameters, ethanol concentration, microwave power level, and irradiation/extraction time were significant, whereas, feed-to-solvent ratio was insignificant. Therefore, antioxidants from the ethanolic extraction leaves of A. sesquipedalis using microwave technique are significantly affected by ethanol concentration, irradiation time and microwave power.