Single residue substitution at N-terminal affects temperature stability and activity of L2 lipase

• Main Authors: Bukhari, Noramirah, Leow, Adam Thean Chor, Raja Abdul Rahman, Raja Noor Zaliha, Mohd Shariff, Fairolniza
• Format: Article
• Published: MDPI AG 2020
• Online Access: http://psasir.upm.edu.my/id/eprint/85827/; https://www.mdpi.com/1420-3049/25/15/3433
• Institution: Universiti Putra Malaysia

Rational design is widely employed in protein engineering to tailor wild-type enzymes for industrial applications. The typical target region for mutation is a functional region like the catalytic site to improve stability and activity. However, few have explored the role of other regions which, in p...