Use of reverse transcription-polymerase chain reaction (RT-PCR) for Cymbidium mosaic virus (CyMV) detection in orchids

• Main Author: Mat, Mazidah
• Format: Thesis
• Language: English; English
• Published: 2000
• Subjects: Cymbidium mosaic virus; Polymerase chain reaction - Diagnostic use
• Online Access: http://psasir.upm.edu.my/id/eprint/9129/1/FSAS_2000_33%20IR.pdf; http://psasir.upm.edu.my/id/eprint/9129/
• Institution: Universiti Putra Malaysia

The reverse transcription-polymerase chain reaction CRT-PCR) was adapted for detection of Cymbidium mosaic virus CCyMV) in orchids. The oligonucleotide primers used were selected from the predicted homologous coat protein region of CyMV and other Potexviruses which enabled to amplify approximate...