Optimized green extraction conditions of matcha green tea (Camellia sinensis) using central composite design for maximal polyphenol and antioxidant contents
Response surface methodology (RSM) was employed to optimize the extraction conditions of phenolic and antioxidant compounds from matcha green tea (Camellia sinensis) using central composite design (CCD). The desirability function was used to find the optimum extraction conditions. The highest po...
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| Main Authors: | , , , , |
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| Format: | Article |
| Published: |
North Carolina State University * College of Natural Resources
2021
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| Online Access: | http://psasir.upm.edu.my/id/eprint/94466/ https://bioresources.cnr.ncsu.edu/resources/optimized-green-extraction-conditions-of-matcha-green-tea-camellia-sinensis-using-central-composite-design-for-maximal-polyphenol-and-antioxidant-contents/ |
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| Institution: | Universiti Putra Malaysia |
| Summary: | Response surface methodology (RSM) was employed to optimize the
extraction conditions of phenolic and antioxidant compounds from matcha
green tea (Camellia sinensis) using central composite design (CCD). The
desirability function was used to find the optimum extraction conditions.
The highest polyphenol and antioxidant content yield were reached at a
temperature of 80 °C, an extraction time of 20 min, a liquid-to-solid ratio of
100 mL/g, and a desirability value of 0.948. The experimental values for
total phenolics under the optimum extraction conditions were 317.62 ±
3.45 mg GAE/g and 29.21 ± 0.38 mg RE/g for the total flavonoids. The
antioxidant activity (AA) was evaluated using 2,2 diphenyl-1-picrylhydrazyl
(DPPH) and 2,2-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS),
which showed radical scavenging activities at 88.28 ± 0.14% and 90.02 ±
0.14%, respectively. The high performance liquid chromatography (HPLC)
analysis at the optimum condition revealed 14 compounds. Among the
analyzed compounds, matcha green tea extract (MGTE) had the highest
content of epigallocatechin gallate (EGCG) with 95.48 mg/g, followed by
epicatechin gallate (ECG) at 74.48 mg/g, and catechin at 28.94 mg/g. The
results suggested that the optimized parameters of heat-assisted
extraction provide an ideal green extraction method for the extraction of
the high polyphenol and antioxidant content in matcha green tea. |
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