Establishment Of A Zebrafish Embryonic Stem Cell Line Using A Feeder-Free Culture Method And In Vitro Pluripotency Characterization
Feeder layers are commonly used in zebrafish embryonic stem (ES) cell cultures to enable proliferation and prevent differentiation of the ES cells. In this study, a culture method using feeder-free was employed to derive and establish longterm zebrafish ES cell line from zebrafish blastula stage...
Saved in:
| Main Author: | |
|---|---|
| Format: | Thesis |
| Language: | English |
| Published: |
2014
|
| Subjects: | |
| Online Access: | http://eprints.usm.my/29097/1/Establishment_of_a_zebrafish_embryonic_stem_cell_line_using_a_feeder-free_culture_method_and_in_vitro_puripotency_characterization.pdf http://eprints.usm.my/29097/ |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| Institution: | Universiti Sains Malaysia |
| Language: | English |
| Summary: | Feeder layers are commonly used in zebrafish embryonic stem (ES) cell
cultures to enable proliferation and prevent differentiation of the ES cells. In this
study, a culture method using feeder-free was employed to derive and establish longterm
zebrafish ES cell line from zebrafish blastula stage embryos.
Lapisan sel penyuap telah biasa digunakan dalam kultur sel tunjang
embrionik daripada ikan zebra untuk membolehkan proliferasi dan mencegah
pembezaan sel-sel tersebut. Dalam kajian ini, kaedah kultur tanpa lapisan sel
penyuap telah diguna untuk menghasilkan titisan sel tunjang embrionik jangka masa
panjang daripada embrio peringkat blastula ikan zebra. |
|---|