Establishment Of A Zebrafish Embryonic Stem Cell Line Using A Feeder-Free Culture Method And In Vitro Pluripotency Characterization

Feeder layers are commonly used in zebrafish embryonic stem (ES) cell cultures to enable proliferation and prevent differentiation of the ES cells. In this study, a culture method using feeder-free was employed to derive and establish longterm zebrafish ES cell line from zebrafish blastula stage...

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Main Author: Ho, Sing Yee
Format: Thesis
Language:English
Published: 2014
Subjects:
Online Access:http://eprints.usm.my/29097/1/Establishment_of_a_zebrafish_embryonic_stem_cell_line_using_a_feeder-free_culture_method_and_in_vitro_puripotency_characterization.pdf
http://eprints.usm.my/29097/
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Institution: Universiti Sains Malaysia
Language: English
id my.usm.eprints.29097
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spelling my.usm.eprints.29097 http://eprints.usm.my/29097/ Establishment Of A Zebrafish Embryonic Stem Cell Line Using A Feeder-Free Culture Method And In Vitro Pluripotency Characterization Ho, Sing Yee G Geography (General) SF Animal culture Feeder layers are commonly used in zebrafish embryonic stem (ES) cell cultures to enable proliferation and prevent differentiation of the ES cells. In this study, a culture method using feeder-free was employed to derive and establish longterm zebrafish ES cell line from zebrafish blastula stage embryos. Lapisan sel penyuap telah biasa digunakan dalam kultur sel tunjang embrionik daripada ikan zebra untuk membolehkan proliferasi dan mencegah pembezaan sel-sel tersebut. Dalam kajian ini, kaedah kultur tanpa lapisan sel penyuap telah diguna untuk menghasilkan titisan sel tunjang embrionik jangka masa panjang daripada embrio peringkat blastula ikan zebra. 2014 Thesis NonPeerReviewed application/pdf en http://eprints.usm.my/29097/1/Establishment_of_a_zebrafish_embryonic_stem_cell_line_using_a_feeder-free_culture_method_and_in_vitro_puripotency_characterization.pdf Ho, Sing Yee (2014) Establishment Of A Zebrafish Embryonic Stem Cell Line Using A Feeder-Free Culture Method And In Vitro Pluripotency Characterization. Masters thesis, Universiti Sains Malaysia.
institution Universiti Sains Malaysia
building Hamzah Sendut Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Sains Malaysia
content_source USM Institutional Repository
url_provider http://eprints.usm.my/
language English
topic G Geography (General)
SF Animal culture
spellingShingle G Geography (General)
SF Animal culture
Ho, Sing Yee
Establishment Of A Zebrafish Embryonic Stem Cell Line Using A Feeder-Free Culture Method And In Vitro Pluripotency Characterization
description Feeder layers are commonly used in zebrafish embryonic stem (ES) cell cultures to enable proliferation and prevent differentiation of the ES cells. In this study, a culture method using feeder-free was employed to derive and establish longterm zebrafish ES cell line from zebrafish blastula stage embryos. Lapisan sel penyuap telah biasa digunakan dalam kultur sel tunjang embrionik daripada ikan zebra untuk membolehkan proliferasi dan mencegah pembezaan sel-sel tersebut. Dalam kajian ini, kaedah kultur tanpa lapisan sel penyuap telah diguna untuk menghasilkan titisan sel tunjang embrionik jangka masa panjang daripada embrio peringkat blastula ikan zebra.
format Thesis
author Ho, Sing Yee
author_facet Ho, Sing Yee
author_sort Ho, Sing Yee
title Establishment Of A Zebrafish Embryonic Stem Cell Line Using A Feeder-Free Culture Method And In Vitro Pluripotency Characterization
title_short Establishment Of A Zebrafish Embryonic Stem Cell Line Using A Feeder-Free Culture Method And In Vitro Pluripotency Characterization
title_full Establishment Of A Zebrafish Embryonic Stem Cell Line Using A Feeder-Free Culture Method And In Vitro Pluripotency Characterization
title_fullStr Establishment Of A Zebrafish Embryonic Stem Cell Line Using A Feeder-Free Culture Method And In Vitro Pluripotency Characterization
title_full_unstemmed Establishment Of A Zebrafish Embryonic Stem Cell Line Using A Feeder-Free Culture Method And In Vitro Pluripotency Characterization
title_sort establishment of a zebrafish embryonic stem cell line using a feeder-free culture method and in vitro pluripotency characterization
publishDate 2014
url http://eprints.usm.my/29097/1/Establishment_of_a_zebrafish_embryonic_stem_cell_line_using_a_feeder-free_culture_method_and_in_vitro_puripotency_characterization.pdf
http://eprints.usm.my/29097/
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