Mengenalpasti fenotip apolipoprorein E dengan menggunakan teknik fokus isoelektrik bagi kes-kes hiperlipidemia di Kelantan

• Main Authors: Rashid, Faridah Abdul, Mansur, Mazidah Ahmad, Nordin, Hasenan
• Format: Conference or Workshop Item
• Language: English
• Published: 1999
• Subjects: RC Internal medicine
• Online Access: http://eprints.usm.my/42376/1/GP...Mengenalpasti_Fenotip_Apolipoprotein_E_Dengan_Menggunakan_Teknik_Fokus_Isoelektrik_Bagi_Kes-Kes_Hiperlipidemia_Di_Kelantan...OCR.pdf; http://eprints.usm.my/42376/
• Institution: Universiti Sains Malaysia

Patients plasma samples (n=250) were screened for cholesterol and triglyceride content by enzymatic analyses performed on an automated chemistry analyzer using standard kits. Lipoprotein electrophoresis was performed on thin slab or pre-casted tube gels.Unrelated plasma were selected (n=24) and studied using both gel methods. One subject with a possible apolipoprotein E-2/2 phenotype was detected by the slab gel method. Another 11 subjects with unusual lipoprotein banding patterns and possible altered apolipoprotein E phenotypes were detected by the tube gel method. VLDL were isolated by a micromethod developed on an ultracentrifuge. Apolipoprotein E phenotype was confirmed by flat bed isoelectrtc focussing (IEF) of delipidated VLDL proteins on commercial and homemade IEF gels.