Overexpression, purification and characterization of the Aspergillus niger endoglucanase, EglA, in Pichia pastoris

• Main Authors: Quay, D. H. X., Bakar, F. D. A., Rabu, A., Said, M., Md. Illias, Rosli, Mahadi, N. M., Hassan, O., Murad, A. M. A.
• Format: Article
• Published: Academic Journals 2011
• Subjects: QD Chemistry; TA Engineering (General). Civil engineering (General)
• Online Access: http://eprints.utm.my/id/eprint/29557/; https://academicjournals.org/journal/AJB/article-abstract/3F357DD31277
• Institution: Universiti Teknologi Malaysia

Cellulases are industrially important hydrolytic enzymes applicable in the bioconversion of cellulosic biomass to simple sugars. In this work, an endoglucanase from Aspergillus niger ATCC 10574, EglA, was expressed in the methylotrophic yeast Pichia pastoris and the properties of the recombinant pro...