Determination of tamoxifen and its metabolites using micelle to solvent stacking in nonaqueous capillary electrophoresis

• Main Authors: Thang, Lee Yien, See, Hong Heng, Quirino, Joselito P.
• Format: Article
• Published: Wiley-VCH Verlag 2016
• Subjects: QD Chemistry
• Online Access: http://eprints.utm.my/id/eprint/69173/; http://dx.doi.org/10.1002/elps.201600010
• Institution: Universiti Teknologi Malaysia

Micelle to solvent stacking was implemented for the recently established NACE-C4D method to determine tamoxifen and its metabolites in standard samples and human plasma of breast cancer patients. For stacking, the standard samples and extract after liquid-liquid extraction (LLE) were prepared in methanol and the resulting sample solution was pressure injected after a micellar plug of SDS. Factors that affected the stacking such as SDS concentration, micelle, and sample plug length were examined. The sensitivity enhancement factor (peak height from stacking/peak height from typical injection of sample in BGE) was 15-22. The method detection limits with LLE were in the range of 5-10 ng/mL, which was lower than the established method (where the LLE extract was also prepared in methanol) with reported method detection limits of 25-40 ng/mL. The intraday and interday repeatability were in the range of 1.0-3.4% and 3.8-6.5%, respectively.